MRI: Acquisition of a Ultra-High Resolution Fourier Transform Ion Cyclotron Resonance (FTICR) Mass Spectrometer for the Georgia Institute of Technology Core Facilities

MRI：为佐治亚理工学院核心设施购置超高分辨率傅里叶变换离子回旋共振 (FTICR) 质谱仪

• 批准号: 1726528
• 负责人: Facundo Fernandez
• 金额: $ 100.92万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726528&HistoricalAwards=false
• 关键词: MRI; Acquisition; Ultra; Resolution; Fourier

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation Programs. Professor Facundo Fernandez from Georgia Institute of Technology and colleagues Julia Kubanek, Nga Lee Ng and Matthew Torres are acquiring a high resolution Fourier-transform ion-cyclotron resonance mass spectrometer (HRes FTICR-MS) with ESI (electrospray ionization), MALDI (matrix-assisted laser desorption), ECD and EDT (electron-capture dissociation and electron-transfer dissociation), CID (collision induced dissociation) and imaging capabilities. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. In a typical experiment, the components flow into a mass spectrometer where they are ionized and the ion masses are measured. This highly sensitive technique allows detection of molecules and determination of their structure even when present in a complex mixture. The acquisition strengthens the research infrastructure at the university and regional area. The instrument broadens participation by involving diverse students in research and training using this modern analytical technique. It also provides training opportunities to many undergraduate, graduate and postdoctoral students as well as high school students through a variety of programs such as Engaging New Generations at Georgia Tech through Engineering and Science (ENGAGES), a program that is directed towards underrepresented students in the Atlanta area. This advanced mass spectrometer serves as a regional resource through collaborations involving multiple institutions such as Emory University, Spelman College, Morehouse College, Clark Atlanta University, Georgia State University, the Centers for Disease Control and Prevention, and other institutions.This mass spectrometer enhances research and education at all levels. It benefits researchers studying proto-peptide evolving systems: post-translational modifications in large, intact, G-protein coupled receptors and their associated G proteins. The instrumentation is also used in investigations of metabolomics and lipidomics, and in developing 3-dimensional surface analysis tools and applications that may be useful to follow brain changes after traumatic brain injuries. The mass spectrometer benefits research in cell manufacturing, synthetic phage modification, nanoparticle metabolomics, and glycoproteomics. In addition, the instrument is used for research studies of photosynthetic electron transfer, quorum sensing, food-disinfection by-products, protein trafficking, and nanomaterials.

该奖项由主要研究仪器（MRI）和化学研究仪器计划支持。来自格鲁吉亚理工学院的Facundo费尔南德斯教授及其同事Julia Kubanek、Nga Lee Ng和Matthew托雷斯正在获得一台高分辨率傅里叶变换离子回旋共振质谱仪（HRes FTICR-MS），该质谱仪具有ESI（电喷雾电离）、MALDI（基质辅助激光解吸）、ECD和EDT（电子捕获解离和电子转移解离）、CID（碰撞诱导解离）和成像功能。一般来说，质谱（MS）是用于识别和表征复杂样品中少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离并测量离子质量。这种高灵敏度的技术允许检测分子并确定其结构，即使存在于复杂的混合物中。 此次收购加强了大学和地区的研究基础设施。该仪器通过让不同的学生参与使用这种现代分析技术的研究和培训来扩大参与。它还提供了培训机会，许多本科生，研究生和博士后学生以及高中学生通过各种方案，如通过工程和科学（ENGAGES）在格鲁吉亚技术吸引新一代，这是针对亚特兰大地区代表性不足的学生的方案。这台先进的质谱仪通过与埃默里大学、斯佩尔曼学院、莫尔豪斯学院、克拉克亚特兰大大学、格鲁吉亚州立大学、疾病控制和预防中心等多个机构的合作，成为一种区域性资源，增强了各级研究和教育。它有利于研究原肽进化系统的研究人员：在大的，完整的，G蛋白偶联受体及其相关的G蛋白的翻译后修饰。该仪器还用于代谢组学和脂质组学的研究，以及开发三维表面分析工具和应用程序，这些工具和应用程序可能有助于跟踪创伤性脑损伤后的大脑变化。质谱仪有益于细胞制造、合成噬菌体修饰、纳米颗粒代谢组学和糖蛋白组学的研究。此外，该仪器还用于光合电子转移、群体感应、食品消毒副产品、蛋白质运输和纳米材料的研究。