Profiling tissue protein, elemental ions and nanoparticle distributions

分析组织蛋白质、元素离子和纳米颗粒分布

• 批准号: DP160102063
• 负责人: Prof Paul Witting
• 金额: $ 24.18万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160102063
• 关键词: Profiling; tissue; protein; elemental; ions

This project aims to investigate protein-protein interactions that are crucial to homeostatic cell signalling and viability in a changing tissue environment. The central goal is to develop and validate protocols to combine cutting-edge tissue imaging modalities to map and characterise tissue distributions of native and modified proteins, elemental ions and pharmacological agents including nanoparticles and nanovehicles. The aim is to use novel tissue scanning mass spectrometry techniques in conjunction with X-ray-based microprobe spectroscopy and advanced multi-parameter cytometry to identify spatial distributions of proteins, ions and drugs in tissues. This approach may provide new information about the maintenance of homeostatic control and the content, distribution and potential metabolism of drugs or nanoparticles within biological tissues.

该项目旨在研究蛋白质-蛋白质相互作用，这些相互作用对于在不断变化的组织环境中保持细胞信号和活力至关重要。中心目标是开发和验证协议，以结合联合收割机尖端组织成像模式，绘制和分析天然和修饰蛋白质、元素离子和药理学试剂（包括纳米颗粒和纳米载体）的组织分布。其目的是使用新的组织扫描质谱技术，结合基于X射线的微探针光谱和先进的多参数细胞术，以确定组织中蛋白质，离子和药物的空间分布。这种方法可以提供关于维持稳态控制以及药物或纳米颗粒在生物组织内的含量、分布和潜在代谢的新信息。