Engineering aspects for tissue engineering of hyaline cartilage

该申请基于项目“透明软骨组织工程的工程学基础”。自己的结果和文献表明，体外软骨的时空形成还没有被充分了解，特别是与体内软骨的形成相比。在拟议的项目中，将检查软骨细胞在软骨形成过程中的生理变化程度(代谢活动、基因表达、蛋白质表达)和基质特性(成分、生物力学特性)是否能够被检测和量化，并相互关联。特别是，将讨论是否要从细胞分化状态的改变和/或细胞营养供应和代谢物清除不足的情况来寻找原因。然后，这个概念可以用来推导出改进种植策略的方法。

The application is based on the project "Engineering aspects for tissue engineering of hyaline cartilage". Own results and literature show that the temporal and spatial formation of cartilage in vitro is not yet sufficiently understood, especially in comparison to cartilage formation in vivo. Within the proposed project it will be examined, whether the extent of physiological changes in the cartilage cells during cartilage formation (metabolic activity, gene expression, protein expression) and matrix properties (composition, biomechanical properties) can be detected and quantified, and related to each other. In particular, the question will be addressed whether the reasons for this are to be sought in a change in the state of differentiation of cells and / or an inadequate nutrient supply and metabolite removal of the cells. This concept can then be used to derive approaches for improved cultivation strategies.