Engineering aspects for tissue engineering of hyaline cartilage

该应用是基于项目“透明软骨组织工程的工程方面”。自身的结果和文献表明，体外软骨的时间和空间形成尚未得到充分的了解，特别是与体内软骨形成相比。在拟议的项目中，将检查软骨细胞在软骨形成过程中的生理变化程度（代谢活性，基因表达，蛋白质表达）和基质特性（组成，生物力学特性）是否可以检测和量化，并相互关联。特别是，这个问题将被解决的原因是否要在细胞分化状态的变化和/或营养供应不足和细胞的代谢物去除中寻找。这个概念可以用来推导出改进栽培策略的方法。

The application is based on the project "Engineering aspects for tissue engineering of hyaline cartilage". Own results and literature show that the temporal and spatial formation of cartilage in vitro is not yet sufficiently understood, especially in comparison to cartilage formation in vivo. Within the proposed project it will be examined, whether the extent of physiological changes in the cartilage cells during cartilage formation (metabolic activity, gene expression, protein expression) and matrix properties (composition, biomechanical properties) can be detected and quantified, and related to each other. In particular, the question will be addressed whether the reasons for this are to be sought in a change in the state of differentiation of cells and / or an inadequate nutrient supply and metabolite removal of the cells. This concept can then be used to derive approaches for improved cultivation strategies.