Engineering aspects for tissue engineering of hyaline cartilage

透明软骨组织工程的工程方面

• 批准号: 231082117
• 负责人: Professor Dr. Michael Amling
• 金额: --
• 依托单位: Institut für Osteologie und Biomechanik (IOBM)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/231082117?language=en
• 关键词: Engineering; aspects; tissue; engineering; hyaline

The application is based on the project "Engineering aspects for tissue engineering of hyaline cartilage". Own results and literature show that the temporal and spatial formation of cartilage in vitro is not yet sufficiently understood, especially in comparison to cartilage formation in vivo. Within the proposed project it will be examined, whether the extent of physiological changes in the cartilage cells during cartilage formation (metabolic activity, gene expression, protein expression) and matrix properties (composition, biomechanical properties) can be detected and quantified, and related to each other. In particular, the question will be addressed whether the reasons for this are to be sought in a change in the state of differentiation of cells and / or an inadequate nutrient supply and metabolite removal of the cells. This concept can then be used to derive approaches for improved cultivation strategies.

该应用程序是基于项目“透明软骨组织工程的工程方面”。自己的研究结果和文献表明，软骨在体外的时间和空间形成还没有得到充分的理解，特别是在体内软骨形成相比。在拟议的项目中，将检查软骨形成过程中软骨细胞的生理变化程度（代谢活性，基因表达，蛋白质表达）和基质性质（组成，生物力学性质）是否可以检测和量化，并相互关联。具体而言，将解决的问题是，是否要在细胞分化状态的变化和/或细胞的营养供应和代谢物去除不足中寻找其原因。然后，可以使用这个概念来推导出改进栽培策略的方法。