SBIR Phase I: Remediation of recalcitrant and emerging environmental organic contaminants of concern using bacterial approaches

SBIR 第一阶段：使用细菌方法修复令人关注的顽固性和新出现的环境有机污染物

• 批准号: 1746882
• 负责人: Raymond Sambrotto
• 金额: $ 22.5万
• 依托单位: ALLIED MICROBIOTA LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1746882&HistoricalAwards=false
• 关键词: SBIR; Phase; Remediation; recalcitrant; emerging

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) project addresses the critical societal need to remediate widespread problems of toxic organic pollutants in soils, sediments and groundwater. The technology results in a significantly improved ability to destroy organic environmental contaminants by reducing treatment time and costs at commercial scales using novel bacterial strains. These strains have a demonstrated ability to rapidly degrade a variety of important, recalcitrant organic pollutants including polychlorinated biphenyls (PCBs), polyaromatic hydrocarbons (PaHs) and dioxins. This project will assess the costs and efficacy of the large-scale implementation of this technology that includes the incubation of the contaminated material at elevated temperatures (60¢ªC/150¢ªF) to enhance degradation. This enables environmental goals to be met on many sites whose cleanup is currently limited by the high costs of remediation. The commercial impact is significant and makes possible the re-development of many billions of dollars of real estate in these categories to return these properties to productive and appropriate use. Additional aspects of the technology are applicable to emerging pollutants such as 1,4-dioxane in groundwater and greatly expand the options available to environmental engineers and landowners for cleaning up long-lived pollutants. This SBIR Phase I project proposes to develop two novel modes of destroying organic contaminants of concern that reduce remediation costs and improve outcomes. The project improves on prior approaches to bioremediation by using heat-tolerant bacteria for faster rates of chemical destruction for a broad range of pollutants. Objectives include the evaluation of the efficacy and costs to treat large amounts (5-10 tons) of material with whole bacteria as basis to assess the commercial application of the technology. This involves several components, including the optimization of the large-scale cell production protocols to produce the bacteria. In conjunction with environmental engineering firms, the project also will develop cost-effective approaches to maintaining the appropriate temperature, moisture and oxygen conditions for optimal cell growth during large-scale treatments. Variations in incubation conditions and flocculent additions will be used to determine the lowest pollutant levels that can be attained. Finally, a new approach using cell extracts instead of live cells will be evaluated for both soils and sediments as well as for soluble pollutants like 1,4-dioxane. These approaches will provide critical data on remediation costs at commercial scale and also evaluate new approaches to emerging groundwater pollutants that are difficult to remediate with current technology.

这个小企业创新研究（SBIR）项目的更广泛影响/商业潜力解决了解决土壤、沉积物和地下水中有毒有机污染物广泛问题的紧迫社会需求。 该技术通过使用新型细菌菌株在商业规模上减少处理时间和成本，导致破坏有机环境污染物的能力显著提高。 这些菌株具有快速降解多种重要的难降解有机污染物的能力，包括多氯联苯（PCB），多环芳烃（PaHs）和二恶英。 该项目将评估大规模实施这一技术的成本和效率，包括在高温（60 ℃/150 ℉）下培养受污染的材料，以促进降解。 这使得环境目标能够在许多场地上实现，这些场地的清理目前受到补救费用高昂的限制。商业上的影响是巨大的，使这些类别中数十亿美元的真实的房地产的重新开发成为可能，使这些财产恢复生产和适当的用途。 该技术的其他方面适用于地下水中的1，4-二氧六环等新出现的污染物，并大大扩大了环境工程师和土地所有者清理长期污染物的选择。该SBIR第一阶段项目提出开发两种新的模式来破坏所关注的有机污染物，从而降低修复成本并改善结果。 该项目改进了以前的生物修复方法，使用耐热细菌对广泛的污染物进行更快的化学破坏。 目的包括评价用全菌处理大量（5-10吨）材料的效率和成本，作为评估该技术的商业应用的基础。 这涉及几个组成部分，包括优化大规模细胞生产方案以生产细菌。 该项目还将与环境工程公司合作，开发具有成本效益的方法，以保持适当的温度，湿度和氧气条件，以便在大规模处理期间实现最佳细胞生长。 培养条件和絮凝剂添加量的变化将用于确定可达到的最低污染物水平。 最后，将评估一种使用细胞提取物而不是活细胞的新方法，用于土壤和沉积物以及1，4-二氧六环等可溶性污染物。 这些方法将提供关于商业规模补救费用的关键数据，并评估处理目前技术难以补救的新出现的地下水污染物的新方法。