EAGER: Developing Chemical Probe Technologies to Explore Glycolipid Exchange Between Camellia sinesis (Tea) and Associated Microbes

EAGER：开发化学探针技术来探索茶树（茶）和相关微生物之间的糖脂交换

• 批准号: 1758530
• 负责人: Jacquelyn Gervay-Hague
• 金额: $ 30万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2022-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1758530&HistoricalAwards=false
• 关键词: EAGER; Developing; Chemical; Probe; Technologies

With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Dr. Jacquelyn Gervay-Hague from The University of California at Davis to study plant and microbe interactions important for the sustainable growth of Camellia sinensis (Tea). Soil microorganisms contribute to the health of plants by breaking down organic matter and providing essential nutrients such as phosphorous and nitrogen. This natural exchange is not well understood, partly due to the complexity of farm settings, which host a wide variety of microorganisms. The tissue culture methods and innovations in chemical probe synthesis advanced in this project simplify the problem by focusing on the chemical actors involved, rather than vast numbers of microorganisms. Student and postdoctoral trainees propagate tea plants in environmentally controlled microbe-free environments where all chemical inputs are measured. Plants growing in this setting are fed chemical compounds that are designed for easy isolation and identification of transformed substances. Plants growing in natural settings are also exposed to the same probes and integrated data analyses reveal differences between these environments. Together, these studies provide a chemical basis for characterizing microbial contributions to the health and sustainability of tea plants. Tea is the most widely consumed beverage in the world, besides water. It is a multi-billion dollar import business in the US, where less than 100 acres are currently farmed. The potential for US-grown tea represents an important economic opportunity, if sustainable growing methods can be established. Through the UC Davis Global Tea Initiative, the results of these studies will be communicated to the Global Tea community as well as US-based tea farmers who are invited for field days and seminars in order to maximize knowledge transfer to the farming community.This research undertakes micro-propagation of Camellia sinensis (tea) to establish microbe-free tissue culture (TC) specimens for chemical profiling studies. Various growth stages of aseptic TC are profiled and compared to clones being grown in natural environments with exposure to microorganisms. The plants are fed chemically synthesized probes that are functionalized to enable metabolic pull-down with high sensitivity mass spectrometry and nuclear magnetic resonance detection. Trainees learn both targeted and untargeted analysis to differentiate plant and microbial metabolic pathways. Integrated data analyses, including principal component analysis, is implemented to reveal important metabolic relationships and to provide knowledge for developing synergistic and beneficial plant and microbial environments that promote the growth, health and sustainability of US grown tea.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

通过这一奖项，化学部生命过程化学项目资助加州大学戴维斯分校的Jacquelyn Gervay-海牙博士研究对茶树可持续生长至关重要的植物和微生物相互作用。土壤微生物通过分解有机物和提供磷和氮等基本营养物质来促进植物的健康。这种自然的交换还没有被很好地理解，部分原因是农场环境的复杂性，那里有各种各样的微生物。该项目中提出的组织培养方法和化学探针合成方面的创新通过关注涉及的化学行为者而不是大量的微生物来简化问题。学生和博士后受训人员在环境受控的无微生物环境中繁殖茶树，在那里所有的化学输入都要进行测量。在这种环境下生长的植物会被喂入化合物，这些化合物旨在容易地分离和识别转化的物质。生长在自然环境中的植物也暴露在相同的探测器下，综合数据分析揭示了这些环境之间的差异。总之，这些研究为确定微生物对茶树健康和可持续性的贡献提供了化学基础。除水外，茶是世界上消费最广泛的饮料。在美国，这是一项价值数十亿美元的进口业务，目前美国的耕种面积不到100英亩。如果能够建立可持续的种植方法，美国产茶叶的潜力代表着一个重要的经济机会。通过加州大学戴维斯分校的全球茶叶倡议，这些研究的结果将被传达给全球茶叶社区以及被邀请参加田间活动和研讨会的美国茶农，以最大限度地将知识转移到农业社区。这项研究进行了茶树的微繁殖，以建立用于化学图谱研究的无微生物组织培养(TC)标本。无菌TC的不同生长阶段被描绘出来，并与在自然环境中生长的暴露在微生物中的克隆进行比较。这些植物被给予化学合成的探针，这些探针被功能化，以实现高灵敏度的质谱学和核磁共振检测的代谢下拉。学员学习目标分析和非目标分析，以区分植物和微生物的代谢途径。实施了包括主成分分析在内的综合数据分析，以揭示重要的代谢关系，并为开发协同和有益的植物和微生物环境提供知识，以促进美国种植茶叶的生长、健康和可持续发展。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。