Functional analysis of membrane remodeling by epsin1 (B09)

这个项目的主要目标是从分子上了解内吞蛋白Epsin1如何与膜相互作用，以及这种蛋白质-膜相互作用如何影响膜的形态。使用纯化的蛋白质和不同的模型膜系统，分析脂组成对膜重塑的重要性。我们将研究已知的Epsin1相互作用伙伴对膜变形的调节作用，并将在体外重组Epsin1依赖的货物分类，以了解网状蛋白介导的内吞作用的关键步骤。

The main goal of this project is a molecular understanding of how the endocytic protein epsin1 interacts with membranes and how this protein-membrane interaction affects membrane morphology. Using purified proteins and different model membrane systems the importance of the lipid composition for membrane remodeling will be analyzed. We will study the regulatory effect of known epsin1 interaction partners on membrane deformation, and epsin1 dependent cargo sorting will be reconstituted in vitro to understand key steps of clathrin-mediated endocytosis.