Understanding Molecular-Recognition Properties of Helical Pores under Non-equilibrium Conditions

了解非平衡条件下螺旋孔的分子识别特性

• 批准号: 1905094
• 负责人: Bing Gong
• 金额: $ 22万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1905094&HistoricalAwards=false
• 关键词: Understanding; Molecular; Recognition; Properties; Helical

Professor Bing Gong of the State University of New York at Buffalo and Professor Xiao Cheng Zeng of the University of Nebraska-Lincoln collaborate to develop novel approaches to sensing differences among sugar molecules. In this project, the team designs and synthesizes long-chain molecules that coil up like springs. The interior space of these "molecular springs" interact with different kinds of sugar molecules with different strengths. By embedding these "molecular springs" in a cell wall and monitoring the passage of sugar molecules through the interior spaces, the research team aims to develop a method to recognize and sense sugars in water. The results of this research may have implications in separations and medical diagnostics. The broader impacts involve training graduate and undergraduate students, incorporating research into teaching, conducting workshops, reaching out to underrepresented groups, and broadly disseminating this work to the scientific community. With the support from the Macromolecular, Supramolecular and Nanochemistry Program of the Chemistry Division, this project aims to probe and understand molecular recognition of sugar and sugar alcohols in aqueous media under non-equilibrium conditions. The research team prepares aromatic oligoamide foldamers that form helical coils. When embedded in membranes, the oxygen-rich, electrostatically negative inner pores of these coils provide thruways for ions to pass through the membrane. In addition, the presence of numerous amide oxygen atoms in the inner pores of the membrane-bound foldamers serve as hydrogen-bond acceptors that enable multiple hydrogen-bonding interactions with sugars and sugar alcohols in different strengths. Such interactions, being dynamic and transient, especially in water, result in the perturbation of ion currents through the transmembrane pores. Depending on their structures and properties, different types of carbohydrates perturb the flow of ions through the pores differently, which can be detected very sensitively. By measuring the changes in transmembrane ion conductance in the presence of different saccharides and their derivatives, the research aims to monitor and understand molecular recognition of these molecules under non-equilibrium conditions. Specifically, this work focuses on synthesizing oligoamides of different lengths (8mer, 16mer, 32mer, and 64mer) and examining the effects of various sugars and sugar alcohols on the transmembrane ion currents through the pore-forming foldamers. Vesicle-based assays are performed to provide a rapid but qualitative screening of the effects of chosen carbohydrates, followed by measurement of single-channel currents to reveal the signatures of different carbohydrate molecules.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

纽约州立大学布法罗分校的龚兵教授和内布拉斯加州大学林肯分校的肖承曾教授合作开发了感知糖分子差异的新方法。在这个项目中，该团队设计并合成了像弹簧一样卷曲的长链分子。这些“分子弹簧”的内部空间与不同强度的不同糖类分子相互作用。通过将这些“分子弹簧”植入细胞壁，并监测糖分子通过内部空间的通道，研究小组旨在开发一种识别和感知水中糖的方法。这项研究的结果可能会对分居和医学诊断产生影响。更广泛的影响包括培训研究生和本科生，将研究纳入教学，举办研讨会，接触代表性不足的群体，并向科学界广泛传播这项工作。在化学系大分子、超分子和纳米化学计划的支持下，本项目旨在探索和理解非平衡条件下糖和糖醇在水介质中的分子识别。该研究小组制备了形成螺旋卷曲的芳香族低聚低聚酰胺。当嵌入到膜中时，这些线圈的富氧、静电负的内孔为离子通过膜提供了通道。此外，在膜结合的折叠体的内孔中存在大量的酰胺氧原子，作为氢键受体，使不同强度的糖和糖醇发生多重氢键作用。这种相互作用是动态的和瞬时的，特别是在水中，导致离子电流通过跨膜孔的扰动。根据碳水化合物的结构和性质，不同类型的碳水化合物会以不同的方式扰乱离子通过毛孔的流动，这一点可以非常敏感地检测到。通过测量不同糖及其衍生物存在时跨膜离子电导的变化，本研究旨在监测和了解这些分子在非平衡条件下的分子识别。具体地说，本工作着重于合成不同长度的寡酰胺(8聚体、16聚体、32聚体和64聚体)，并考察各种糖和糖醇对通过成孔折叠体的跨膜离子电流的影响。基于囊泡的分析是为了对所选碳水化合物的影响进行快速但定性的筛选，然后测量单通道电流以揭示不同碳水化合物分子的签名。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Stable pseudo[3]rotaxanes with strong positive binding cooperativity based on shape-persistent aromatic oligoamide macrocycles

基于形状持久的芳香低聚酰胺大环，具有强正结合协同性的稳定假[3]轮烷

• DOI: 10.1039/d1cc05193h
• 发表时间: 2021
• 期刊: Chemical Communications
• 影响因子: 4.9
• 作者: Sobiech, Thomas A.;Zhong, Yulong;Sánchez B., Laura S.;Kauffmann, Brice;McGrath, Jillian K.;Scalzo, Christina;Miller, Daniel P.;Huc, Ivan;Zurek, Eva;Ferrand, Yann
• 通讯作者: Ferrand, Yann

Ultrasensitive liposome-based assay for the quantification of fundamental ion channel properties

基于超灵敏脂质体的测定，用于定量基本离子通道特性

• DOI: 10.1016/j.aca.2020.03.044
• 发表时间: 2020-05-22
• 期刊: ANALYTICA CHIMICA ACTA
• 影响因子: 6.2
• 作者: Shen，Yi;Zhong，Yulong;Shao，Zhifeng
• 通讯作者: Shao，Zhifeng