Collaborative Research: Understanding the Molecular Recognition Behavior of Hollow Helices

合作研究：了解空心螺旋的分子识别行为

• 批准号: 2108538
• 负责人: Bing Gong
• 金额: $ 40万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2108538&HistoricalAwards=false
• 关键词: Collaborative; Research; Understanding; Molecular; Recognition

The Macromolecular, Supramolecular and Nanochemistry Program in the Division of Chemistry is supporting Professor Bing Gong of the State University of New York at Buffalo and Dr. Daniel P. Miller of the Hofstra University to synthesize a series of “host” molecules and investigate their affinity for binding and recognition of sugar molecules. This research aims to gain a better understanding of the factors that will lead to specific interactions with different carbohydrates in order to provide guiding principles for future design of sugar receptors and sensors. This collaborative project integrates experiments with theoretical studies and provides a great platform for research training of undergraduate and graduate students, including students of underrepresented groups. The research results will be incorporated into teaching and broadly disseminated to the scientific community. The host molecules are aromatic oligoamide foldamers with tunable lengths. The oligoamides fold into helical coil structures with multiple hydrogen bond acceptors being directed toward the nanosized hollow core. When embedded in a lipid bilayer membrane in aqueous solution, the helical coils stack to create channels through which ions and small molecules flow from one side of the membrane to the other side. With the numerous H-bonding sites, different sugars and sugar alcohols are expected to interact with the inner pore of the helical coil with different affinities. Such interaction, being dynamic and transient, especially in water, will perturb the ion currents through the transmembrane pores. This research team will (1) optimize the synthesis of the oligoamides, (2) perform one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopic studies and X-ray crystallographic structural analysis to interrogate the interactions between the host and specific carbohydrates to include monosaccharides, disaccharides, and higher oligosaccharides; (3) examine the effects of various carbohydrate guests on the transmembrane ion currents through the helical pores; and (4) carry out computational studies to optimize host-guest binding and to probe the effect carbohydrate guests have on transmembrane ion flows through the helical pores.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

化学系的大分子、超分子和纳米化学项目正在支持纽约州立大学布法罗分校的龚兵教授和霍夫斯特拉大学的丹尼尔·P·米勒博士合成一系列“宿主”分子，并研究它们对糖分子的结合和识别的亲和力。 本研究旨在更好地了解导致与不同碳水化合物发生特定相互作用的因素，以便为未来设计糖受体和传感器提供指导原则。 这个合作项目将实验与理论研究相结合，为本科生和研究生的研究培训提供了一个很好的平台，包括代表性不足的群体的学生。研究成果将纳入教学，并向科学界广泛传播。主体分子是具有可调长度的芳香族低聚酰胺折叠体。 低聚酰胺折叠成螺旋线圈结构，多个氢键受体被导向纳米中空核。 当包埋在水溶液中的脂质双层膜中时，螺旋线圈堆叠以产生通道，离子和小分子通过该通道从膜的一侧流动到另一侧。 由于有大量的氢键位点，不同的糖和糖醇有望以不同的亲和力与螺旋线圈的内孔相互作用。 这种相互作用是动态的和瞬时的，特别是在水中，将扰动通过跨膜孔的离子流。该研究小组将（1）优化低聚酰胺的合成，（2）进行一维和二维核磁共振（NMR）光谱研究和X射线晶体结构分析，以询问宿主与特定碳水化合物之间的相互作用，包括单糖，二糖和高级寡糖;（3）研究了不同碳水化合物客体对跨膜离子流的影响;（4）进行计算研究，以优化主机-客体结合和探测碳水化合物客体对跨膜离子流过螺旋孔的影响。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的学术价值和更广泛的影响审查标准。

项目成果

Ultra‐Tight Host‐Guest Binding with Exceptionally Strong Positive Cooperativity

超紧主客绑定，正协同性极强

• DOI: 10.1002/anie.202213467
• 发表时间: 2022
• 期刊: Angewandte Chemie International Edition
• 作者: Sobiech, Thomas A.;Zhong, Yulong;Miller, Daniel P.;McGrath, Jillian K.;Scalzo, Christina T.;Redington, Morgan C.;Zurek, Eva;Gong, Bing
• 通讯作者: Gong, Bing