Biodegradable and elastic flock scaffolds from a single material system based on chitosan for articular cartilage regeneration

基于壳聚糖的单一材料系统的可生物降解和弹性植绒支架，用于关节软骨再生

• 批准号: 238200731
• 负责人: Professor Dr. Michael Gelinsky
• 金额: --
• 依托单位: Institut für Textilmaschinen und Textile Hochleistungswerkstofftechnik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/238200731?language=en
• 关键词: Biodegradable; elastic; flock; scaffolds; single

The aim of this project is the development of a biodegradable and mechanically stable elastic flock scaffold from a single material system with adjustable parameters based on chitosan as well as the systematic cell biological investigation in regard to its application as three-dimensional carrier structure for tissue engineering of articular cartilage. For this a reproducible spin process for depiction of filament yarns from pure chitosan with defined biological function and properties suitable for textile processing will be developed. Both a chitosan membrane and a novel ultra light surface structured chitosan woven fabric will be developed as substrate (carrier material). A novel adhesive based on chitosan, is applied to join the flock fibers with the substrate. In order to guarantee the electrical conductivity of novel chitosan flock fibers for electrostatic flocking, biocompatible recipe for preparation will be synthesized. A completely biodegradable flock scaffold, which is available as a single material system based on chitosan as substrate, flock fiber and adhesive, is generated by targeted configuration of each component of the flock process. The high demands concerning medical application of the scaffolds require a closed process chain. This will lead to a defined, scalable pore size (preferably 110-150 µm) and simultaneously to a high dimension stability of the scaffolds by minimum material requisition. The anisotropic morphology of the constructs guarantees both mechanical strength, elasticity and a high porosity at the same time.The biocompatibility of all components is proved by cell culture investigations. A continuous and qualified evaluation of the actual work state and a specification of requirements concerning the materials and structures as a fundament for targeted technology and structure development is carried out by means of in vitro tests. Also stability of the flock scaffolds under cell culture conditions and their degradation behavior will e studied. Seeding of biodegradable flock scaffolds with human primary chondrocytes (hCh) and human mesenchymal stem cells (hMSC) and analysis of their chondrogenic differentiation demonstrate the effects of anisotropic pore morphology on the cell behavior. An effective method of cell seeding of these open porous scaffolds will be developed by generating suited biopolymer gels for cultivation of hCh/hMSC on biodegradable flock scaffolds. In additional experiments the effect of cyclic mechanical loading on matrix synthesis and chondrogenic differentiation of cells cultivated in flock scaffolds will be investigated, which will provide the basis for a novel therapy of articular cartilage defects.

本项目旨在以壳聚糖为基础，从单一材料体系中研制一种可生物降解、力学稳定、参数可调的弹性群支架，并对其作为关节软骨组织工程三维载体结构的应用进行系统的细胞生物学研究。为此，将开发一种具有明确生物功能和适合纺织加工性能的纯壳聚糖长丝的可重复纺丝工艺。将开发一种壳聚糖膜和一种新型的超轻表面结构壳聚糖机织物作为基底（载体材料）。采用一种新型的壳聚糖胶粘剂将絮群纤维与基体结合在一起。为保证新型壳聚糖静电植群纤维的导电性，合成了具有生物相容性的制备配方。以壳聚糖为基材，以絮群纤维和黏合剂为基体，通过对絮群过程中各组分的定向配置，生成了一种完全可生物降解的絮群支架。医用支架的高要求需要一个封闭的工艺链。这将导致一个明确的、可扩展的孔径（最好是110-150µm），同时通过最少的材料需求实现支架的高尺寸稳定性。结构的各向异性形态同时保证了机械强度、弹性和高孔隙率。所有组分的生物相容性通过细胞培养调查得到证实。通过体外试验，对实际工作状态进行持续和合格的评估，并制定有关材料和结构的要求规范，作为目标技术和结构开发的基础。此外，还将研究该材料在细胞培养条件下的稳定性及其降解行为。用人原代软骨细胞（hCh）和人间充质干细胞（hMSC）进行生物可降解群支架的播种，并对其软骨分化进行分析，证明了各向异性孔隙形态对细胞行为的影响。通过制备合适的生物聚合物凝胶，在可生物降解的群体支架上培养hCh/hMSC，将开发出一种有效的细胞播种方法。在进一步的实验中，我们将研究循环力学载荷对基质合成和细胞成软骨分化的影响，这将为关节软骨缺损的新疗法提供基础。