MRI: Acquisition of a Leica SP8 WLL SuperResolution and Light Sheet Microscope

授予纽约亨特学院（CUNY）赞助收购一种新的具有超分辨率和数字光片功能的激光扫描共聚焦显微镜（LSM-DLS），该显微镜将被安置在开放访问的多用户生物成像核心设施中。所要求的显微镜将有助于来自多个部门的教师不断增长的研究和教学需求，以及亨特学院，其他CUNY校区和其他区域机构的下一代科学家跨多个学科的培训需求。高中，本科生和研究生将受益于动手培训创新，领先的荧光成像技术，通过课程工作和研究，这将提高他们的教育和指导重要的是他们未来的职业生涯在生物科学。亨特学院一直致力于通过提供卓越和积极的研究环境，增加代表性不足的少数民族研究人员追求科学事业的机会。 已经到位的学院赞助计划鼓励更广泛地参与科学研究，这将通过收购这个国家的最先进的显微镜系统得到加强。新的LSM-DLS将不仅满足当前教师的需求，但也将满足未来的需求学院和大学内的几个部门。它将改变和推进收集定量，高空间和时间分辨率图像的研究能力，以回答细胞生物学，发育生物学，神经科学和生物材料纳米纤维的关键问题。它将支持NSF和其他资助的广泛领域的研究，包括：（a）转录开关，（B）亚细胞分子定位，（c）细胞骨架组织，（d）外泌体功能，（e）髓鞘形成，（f）大脑中树突棘形态动力学。该技术允许对大范围的样品进行可视化，并以高分辨率在真实的时间内检测从单个分子到整个生物体的发育。为了执行这些项目，研究人员将利用LSM-DLS设置的全部功能，包括3D重建，实时成像，光谱解混和光片照明，为他们提供前所未有的能力，以子衍射尺度可视化从无脊椎动物卵母细胞到脊椎动物大脑的结构。这些研究的结果将发表在同行评审的科学期刊上，在科学会议上发表，并用于教学和公共宣传活动。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估来支持。

An award is made to Hunter College of the City University of New York (CUNY) to sponsor the acquisition of a new laser scanning confocal microscope with super-resolution and digital light sheet capabilities (LSM-DLS), which will be housed in the open-access, multi-user Bio-Imaging Core Facility. The requested microscope will assist the growing research and teaching needs of faculty from multiple departments, as well as the training needs of the next generation of scientists across several disciplines at Hunter College, other CUNY campuses, and at other regional institutions. High school, undergraduate and graduate students will benefit from hands- on training in innovative, leading-edge fluorescence imaging techniques through course work and research, which will enhance their education and mentorship important for their future careers in biological sciences. Hunter College has continued commitment to increase the opportunities for under-represented minority researchers to pursue scientific careers by providing an environment of excellence and active research. Already in-place College sponsored programs encourage broader participation in scientific research that will be strengthen by the acquisition of this state-of-the-art microscope system.The new LSM-DLS will not only meet the needs of current faculty, but will also meet future needs of several departments within both the College and University. It will transform and advance the research capabilities for collecting quantitative, highly spatial and temporal resolution images in order to answer critical questions in cell biology, developmental biology, neuroscience, and nanofabrication of biomaterials. It will support NSF- and other funded research in a broad range of areas including: (a) transcriptional switches, (b) sub-cellular molecular localization, (c) cytoskeleton organization, (d) exosome function, (e) myelin formation, and (f) dendritic spine morphology dynamics in the brain. This technology allows for the visualization of a wide range of samples, and detection from single molecules to whole organism development in real time at high resolution. To perform these projects, investigators will utilize the full range of capabilities of the LSM-DLS setup, including 3D reconstruction, live imaging, spectral unmixing and light-sheet illumination, providing them with unprecedented abilities to visualize structures at a sub-diffraction scale from invertebrate oocytes to the vertebrate brain. The results of these studies will be published in scientific peer-reviewed journals, presented at scientific meetings and used in both teaching and public outreach activities.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.