CAREER: Multi-Parametric Electrophysiology for High-Resolution Deep Brain Circuit Analysis

职业：用于高分辨率深部脑回路分析的多参数电生理学

• 批准号: 1944846
• 负责人: Barbara Smith
• 金额: $ 50万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1944846&HistoricalAwards=false
• 关键词: CAREER; Multi; Parametric; Electrophysiology; Resolution

The research goal of this CAREER program is to develop and apply a new technology that integrates photoacoustics (i.e., sound generated by light) and fluorescence to precisely target neurocircuits known to be activated in response to addiction. Identifying the neuronal subtype in reward circuitry is important to understanding addiction. This group will further develop effective pharmacotherapies for targeted treatments. The scientific outcomes of this CAREER project will be specifically incorporated into education and outreach. Through these efforts, new opportunities will be developed for education, community engagement, and motivation of the next generation of medically-inspired engineers.Limitations in optical imaging depth and cellular identification confines targeted high resolution recording of neuronal circuits to the top layer of the living brain. A novel tool, pioneered by the principal investigator, will serve as the foundation of a new scalable and automated approach to measure and control neuronal signals deep in the living brain with high spatial resolution and genetic specificity. This will enable investigators to better characterize and understand complex neural interactions, improving our fundamental understanding of widespread brain circuit activity that will be broadly useful for a variety of neurological disorders and neurodegenerative diseases, beyond current capabilities. Establishing a valid and reliable system to target select cells in the deep brain, precisely modulate neuronal activity, and record electrical signals from multiple neurons within a single circuit will enable transformational discoveries in neuroscience scientificThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项职业计划的研究目标是开发和应用一种集成光声学(即由光产生的声音)和荧光的新技术，以精确定位已知在上瘾时被激活的神经回路。识别奖赏回路中的神经元亚型对于理解成瘾很重要。该小组将进一步开发针对靶向治疗的有效药物疗法。这一职业项目的科学成果将具体纳入教育和外联工作。通过这些努力，将为教育、社区参与和激励下一代医学启发的工程师开发新的机会。光学成像深度和细胞识别的限制将有针对性地将神经元回路的高分辨率记录限制在活着的大脑的顶层。由首席研究人员首创的一种新工具，将作为一种新的可扩展和自动化的方法的基础，以高空间分辨率和遗传特异性测量和控制活着的大脑深处的神经元信号。这将使研究人员能够更好地描述和理解复杂的神经相互作用，提高我们对广泛存在的大脑电路活动的基本理解，这些活动将对各种神经疾病和神经退行性疾病广泛有用，超出了目前的能力。建立一个有效和可靠的系统来瞄准大脑深层的选定细胞，精确调节神经元活动，并在一个电路内记录来自多个神经元的电信号，将使神经科学领域的变革性发现成为可能。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Automated microscope-independent fluorescence-guided micropipette

自动化的不依赖于显微镜的荧光引导微量移液器

• DOI: 10.1364/boe.431372
• 发表时间: 2021
• 期刊: Biomedical Optics Express
• 影响因子: 3.4
• 作者: Miranda, Christopher;Howell, Madeleine R.;Lusk, Joel F.;Marschall, Ethan;Eshima, Jarrett;Anderson, Trent;Smith, Barbara S.
• 通讯作者: Smith, Barbara S.