CSHL Drosophila Neurobiology: Genes, Circuits & Behavior

“果蝇的神经生物学：基因、电路和行为”课程（2020-2023）是一门为期三周的暑期高级强化课程，由具有丰富和互补研究经验的顶尖神经科学家教授，他们研究了这一重要模式生物的大脑功能。本课程由在整个课程期间留在冷泉港实验室（CSHL）的首席讲师和他们实验室的助教讲授。12名学生将从申请者中挑选出来，主要依据是他们的科学价值。课程学员包括高级研究生（通常为70%），博士后学员（通常为25%）和初级教师（5%），他们正在使用苍蝇模型进行神经生物学研究。将积极招募妇女和代表性不足的少数民族。该课程对该领域具有广泛的影响，因为选择学生的部分依据是他们在本国机构传播所学技能和知识的潜力。该课程还为教师、助理和客座讲师提供了在他们的大学里探索果蝇神经科学教学新方法和新材料的机会。课程中制定的协议将通过实验室手册（果蝇神经生物学：实验室手册）的出版和技术视频（将在CSHL的电子技术网络资源上提供）到达课堂以外的受众。该课程由3名课程主任领导的实验课程和多达20名该领域领先专家的日常讲座组成。本课程的实践部分以探究性项目为中心，利用课程中所学到的不同形态和生理测量以及行为范式。具体技术包括分子遗传学分析、定量神经解剖学、计算建模、使用电生理学和遗传编码钙指标记录神经活动、光遗传学和热遗传学控制神经活动，以及许多定量行为测量。客座讲师的演讲将分为两部分。第一部分将介绍他们在自己的研究中使用的核心神经生物学概念和技术，第二部分将重点介绍他们的具体研究如何融入整个领域。总的来说，本课程将全面实用地介绍研究果蝇行为的神经基础的现代实验方法。长期影响分析表明，本课程提高了研究效率，建立了合作关系，并通过传播协议和方法获得了更广泛的受众。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

The "Neurobiology of Drosophila: Genes, Circuits and Behavior" course (2020-2023) is an intensive advanced three week summer course taught by leading neuroscientists with extensive and complementary research experience studying brain function in this important model organism. The course is taught by lead instructors who remain at Cold Spring Harbor Laboratory (CSHL) throughout the duration of the course together with teaching assistants from their laboratories. Twelve students will be selected from a pool of applicants largely on the basis of scientific merit. Course trainees include advanced graduate students (typically 70%), postdoctoral trainees (typically 25%) and junior faculty (5%) who are entering into neurobiological research using the fly model. Women and underrepresented minorities will be actively recruited. The course has a broad impact on the field in that students are chosen based in part on their potential for disseminating the acquired skills and knowledge at their home institutions. The course also provides instructors, assistants and guest lecturers with opportunities to explore new approaches and material for teaching neuroscience with Drosophila at their universities. Protocols developed in the course will reach an audience beyond the classroom through publication of laboratory manuals (Drosophila Neurobiology: A Laboratory Manual) and technique videos that will be made available on CSHL's E-Technical web resource.The course consists of laboratory sessions led by 3 course directors and daily lectures presented by up to 20 leading experts in the field. The hands-on portion of the course is centered on inquiry-based projects, utilizing different morphological and physiological measurements and behavioral paradigms learned in the course. Specific techniques include molecular genetic analyses, quantitative neuroanatomy, computational modeling, recording of neural activity using electrophysiology and genetically encoded calcium indicators, optogenetic and thermogenetic control of neural activity, as well as numerous quantitative behavioral measures. Guest lecturers will divide their presentations into two parts. The first part will address the core neurobiological concepts and techniques they use in their own research, and the second part will focus on how their specific research fits into the field as a whole. Collectively, the course will provide a comprehensive and practical introduction to modern experimental methods for studying the neural basis of behavior in Drosophila. Long-term impact analysis demonstrates that this course enhances research productivity, builds collaborations, and reaches a broader audience through dissemination of protocols and approaches.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.