CSHL Drosophila Neurobiology: Genes, Circuits & Behavior

“果蝇神经生物学：基因、电路和行为”课程（2020-2023年）是一门为期三周的强化高级夏季课程，由领先的神经科学家授课，他们在研究这种重要模式生物的大脑功能方面拥有广泛且互补的研究经验。本课程由在整个课程期间留在冷泉港实验室（CSHL）的首席讲师及其实验室的助教讲授。12名学生将主要根据科学成绩从申请者中选出。课程学员包括高级研究生（通常为70%），博士后学员（通常为25%）和初级教师（5%），他们正在使用苍蝇模型进行神经生物学研究。将积极征聘妇女和代表性不足的少数民族。该课程对该领域产生了广泛的影响，因为选择学生的部分依据是他们在本国机构传播所获得的技能和知识的潜力。该课程还为教师，助理和客座讲师提供机会，探索在大学教授果蝇神经科学的新方法和材料。本课程将通过实验室手册（果蝇神经生物学：实验室手册）和技术视频的出版物（将在CSHL的E-Technical网络资源上提供）向课堂之外的观众提供本课程中开发的协议。本课程包括由3名课程主任领导的实验室课程和由该领域最多20名领先专家提供的每日讲座。该课程的实践部分以探究为基础的项目为中心，利用课程中学到的不同形态和生理测量以及行为范例。具体技术包括分子遗传学分析、定量神经解剖学、计算建模、使用电生理学和遗传编码的钙指标记录神经活动、神经活动的光遗传学和热遗传学控制以及许多定量行为测量。客座讲师将把他们的演讲分为两个部分。第一部分将介绍他们在自己的研究中使用的核心神经生物学概念和技术，第二部分将重点介绍他们的具体研究如何融入整个领域。总的来说，本课程将提供一个全面和实用的介绍现代实验方法研究果蝇行为的神经基础。长期影响分析表明，该课程提高了研究生产力，建立了合作，并通过传播协议和方法达到更广泛的受众。该奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

The "Neurobiology of Drosophila: Genes, Circuits and Behavior" course (2020-2023) is an intensive advanced three week summer course taught by leading neuroscientists with extensive and complementary research experience studying brain function in this important model organism. The course is taught by lead instructors who remain at Cold Spring Harbor Laboratory (CSHL) throughout the duration of the course together with teaching assistants from their laboratories. Twelve students will be selected from a pool of applicants largely on the basis of scientific merit. Course trainees include advanced graduate students (typically 70%), postdoctoral trainees (typically 25%) and junior faculty (5%) who are entering into neurobiological research using the fly model. Women and underrepresented minorities will be actively recruited. The course has a broad impact on the field in that students are chosen based in part on their potential for disseminating the acquired skills and knowledge at their home institutions. The course also provides instructors, assistants and guest lecturers with opportunities to explore new approaches and material for teaching neuroscience with Drosophila at their universities. Protocols developed in the course will reach an audience beyond the classroom through publication of laboratory manuals (Drosophila Neurobiology: A Laboratory Manual) and technique videos that will be made available on CSHL's E-Technical web resource.The course consists of laboratory sessions led by 3 course directors and daily lectures presented by up to 20 leading experts in the field. The hands-on portion of the course is centered on inquiry-based projects, utilizing different morphological and physiological measurements and behavioral paradigms learned in the course. Specific techniques include molecular genetic analyses, quantitative neuroanatomy, computational modeling, recording of neural activity using electrophysiology and genetically encoded calcium indicators, optogenetic and thermogenetic control of neural activity, as well as numerous quantitative behavioral measures. Guest lecturers will divide their presentations into two parts. The first part will address the core neurobiological concepts and techniques they use in their own research, and the second part will focus on how their specific research fits into the field as a whole. Collectively, the course will provide a comprehensive and practical introduction to modern experimental methods for studying the neural basis of behavior in Drosophila. Long-term impact analysis demonstrates that this course enhances research productivity, builds collaborations, and reaches a broader audience through dissemination of protocols and approaches.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.