Collaborative Research: RoL: The intersection between cell fate decisions and phenotypic diversification in a rapidly radiating butterfly lineage
合作研究:RoL:快速辐射蝴蝶谱系中细胞命运决定和表型多样化之间的交叉点
基本信息
- 批准号:2110534
- 负责人:
- 金额:$ 41.07万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-08-15 至 2025-07-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Animal structures are made of combinations and arrangements of distinct cell types, the product of complex cell decision-making during development. But how do cells, which contain identical genetic information, decide their fate? This research addresses this fundamental biological question in a simple, yet spectacularly diverse animal structure–the color patterns on the wings of Heliconius butterflies. Although Heliconius wing patterns are highly diverse, they are created by altering the distribution of just three cell types (red, black and yellow wing scales) across the wing surface. Unlike in a complex organ, the cell decisions that create these patterns unfold on a flat canvas of non-migrating cells. This attribute greatly simplifies the process of understanding the interactions among genes and how these interactions change throughout development to create a specific pattern. This research capitalizes on this fact and emerging genomic tools to characterize the molecular decisions that determine how a developing wing cell becomes specified into one of the three different scale cell types. The project is strengthened by a 6-month internship program that targets traditionally underrepresented groups and offers an in-depth research experience and hands-on professional development. Moreover, through partnerships with science museums, this project will create bilingual (English and Spanish) experiential learning resources that harness the potential of butterflies to educate a variety of audiences (school children, teachers, and life-long learners) about genes, development, natural selection, and the role that interactions among them play in generating Earth’s biodiversity.Evolutionary processes constantly generate and rearrange specialized cell types, forging the morphological dimension of biodiversity. Research is starting to connect changes in gene expression and open chromatin to cell fate decisions. However, this research has mostly focused on early embryonic development or on the developmental trajectories of complex organs in a few species. Although powerful, these studies do not have an explicit goal of linking changes in cell fate decisions to phenotypic change. This research fills this important knowledge gap by characterizing the rules governing cell specification– from signals, to reception, transduction, transcriptional activation, and fate determination during the critical developmental period when the wing patterns of Heliconius butterflies are established. Here, extensive knowledge of the ecological and evolutionary significance of wing color patterns, experimental tractability, and fantastic diversity make Heliconius a powerful experimental system for understanding how the processes of cell specification are modified by natural selection to produce diversity. By casting single-cell transcriptomics, open chromatin profiling and CRISPR loss-of-function experiments within an evolutionary framework that includes replicated cases of the independent evolution of identical wing patterns, this project will determine the rules that govern how cells communicate and acquire a specialized fate during development, and how those rules are applied to generate diversity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
动物的结构是由不同细胞类型的组合和排列组成的,这是发育过程中复杂细胞决策的产物。但是,包含相同遗传信息的细胞如何决定它们的命运呢?这项研究通过一种简单但极其多样化的动物结构--赫利柯斯蝴蝶翅膀上的颜色图案--解决了这个基本的生物学问题。尽管赫利柯斯的翅膀图案非常多样,但它们是通过改变机翼表面上三种细胞类型(红色、黑色和黄色翼鳞)的分布而产生的。与复杂的器官不同,创造这些模式的细胞决定是在一个由非迁移细胞组成的平面画布上展开的。这一属性极大地简化了理解基因之间的相互作用以及这些相互作用在整个发育过程中如何变化以创建特定模式的过程。这项研究利用这一事实和新兴的基因组工具来表征分子决定,这些决定决定了发育中的翼细胞如何被指定为三种不同规模细胞类型之一。该项目通过为期6个月的实习计划得到加强,该计划针对传统上代表性不足的群体,并提供深入的研究经验和亲身实践的专业发展。此外,通过与科学博物馆的伙伴关系,该项目将创建双语(英语和西班牙语)体验式学习资源,利用蝴蝶的潜力教育各种受众(学童、教师和终身学习者)关于基因、发育、自然选择以及它们之间的相互作用在地球生物多样性产生中所起的作用。进化过程不断产生和重新安排专门的细胞类型,锻造生物多样性的形态维度。研究开始将基因表达的变化和开放染色质与细胞命运决定联系起来。然而,这项研究主要集中在少数物种的早期胚胎发育或复杂器官的发育轨迹上。虽然这些研究很强大,但没有明确的目标将细胞命运决定的变化与表型变化联系起来。这项研究通过描述控制细胞规格的规则来填补这一重要的知识空白-从信号到接收、转导、转录激活,以及在蝴蝶翅膀模式建立的关键发育时期的命运决定。在这里,对翅膀颜色图案的生态和进化意义、实验可控性和奇妙的多样性的广泛知识使Helconius成为一个强大的实验系统,用于了解自然选择如何改变细胞指定的过程以产生多样性。通过在进化框架内进行单细胞转录、开放染色质分析和CRISPR功能丧失实验,包括相同翅膀模式的独立进化的复制案例,该项目将确定管理细胞如何在发育过程中沟通和获得特殊命运的规则,以及如何应用这些规则来产生多样性。该奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Arnaud Martin其他文献
Life and Death of Data in Data Lakes: Preserving Data Usability and Responsible Governance
数据湖中数据的生与死:保持数据可用性和负责任的治理
- DOI:
10.1007/978-3-030-34770-3_24 - 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
Marzieh Derakhshannia;C. Gervet;Hicham Hajj;Anne Laurent;Arnaud Martin - 通讯作者:
Arnaud Martin
Genetics of yellow-orange color variation in a pair of sympatric sulfur butterflies.
一对同域硫蝴蝶黄橙色颜色变异的遗传学。
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:8.8
- 作者:
Joseph J. Hanly;C. Francescutti;L. S. Loh;Olaf B W H Corning;Derek J. Long;Marshall A. Nakatani;A. Porter;Arnaud Martin - 通讯作者:
Arnaud Martin
Mapping and CRISPR homology-directed repair of a recessive white eye mutation in emPlodia/em moths
家蚕隐性白眼突变的定位及 CRISPR 同源定向修复
- DOI:
10.1016/j.isci.2022.103885 - 发表时间:
2022-03-18 - 期刊:
- 影响因子:4.100
- 作者:
Christa Heryanto;Joseph J. Hanly;Anyi Mazo-Vargas;Amruta Tendolkar;Arnaud Martin - 通讯作者:
Arnaud Martin
SELP: Semi-supervised evidential label propagation algorithm for graph data clustering
SELP:用于图数据聚类的半监督证据标签传播算法
- DOI:
10.1016/j.ijar.2017.09.008 - 发表时间:
2018 - 期刊:
- 影响因子:3.9
- 作者:
周旷;Arnaud Martin;潘泉;刘准钆 - 通讯作者:
刘准钆
Genetics of continuous colour variation in a pair of sympatric sulphur butterflies
一对同域硫蝴蝶连续颜色变化的遗传学
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:0
- 作者:
Joseph J. Hanly;C. Francescutti;L. S. Loh;Olaf B W H Corning;Derek J. Long;Marshall A. Nakatani;A. Porter;Arnaud Martin - 通讯作者:
Arnaud Martin
Arnaud Martin的其他文献
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{{ truncateString('Arnaud Martin', 18)}}的其他基金
IntBIO: Collaborative Research: Silk Protein Innovation and Novelty (SPIN) : integrating across disciplines to decipher silk fiber evolution
IntBIO:合作研究:丝蛋白创新与新颖(SPIN):跨学科整合,破译丝纤维的进化
- 批准号:
2217156 - 财政年份:2022
- 资助金额:
$ 41.07万 - 项目类别:
Standard Grant
EDGE CT: Precise genome editing in a lepidopteran insect tailored for stable transformation
EDGE CT:鳞翅目昆虫的精确基因组编辑,专为稳定转化而定制
- 批准号:
1923147 - 财政年份:2019
- 资助金额:
$ 41.07万 - 项目类别:
Standard Grant
Collaborative Research: cis-Regulatory Basis of Butterfly Wing Pattern Evolution
合作研究:蝴蝶翅膀图案进化的顺式调控基础
- 批准号:
1656553 - 财政年份:2017
- 资助金额:
$ 41.07万 - 项目类别:
Standard Grant
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