Sustainable bioproduction using phototrophic extracellular electron uptake

利用光养细胞外电子吸收的可持续生物生产

• 批准号: 2021822
• 负责人: Arpita Bose
• 金额: $ 102.93万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2021822&HistoricalAwards=false
• 关键词: Sustainable; bioproduction; using; phototrophic; extracellular

Some bacteria are capable of using electricity and energy from light to fix carbon dioxide and ultimately for renewable, carbon-neutral to carbon-sequestering production of certain high value compounds. The overall goal of this project is to better understand this bioproduction process and to engineer bacteria to use this process for bioplastic and biofuel production. This project will create immersive research opportunities for students at the high school, undergraduate and graduate school levels. Microbial reduction-oxidation reactions drive matter and energy flow in the biosphere. Most microbes use soluble electron donors and acceptors, but some use solid-phase conductive substances. The underlying microbial process is called extracellular electron transfer (EET). The directionality of electron flow during EET can be either outbound, where solid-phase conductive substances are microbially reduced via reductive-EET; or inbound, where solid-phase conductive substances are oxidized via extracellular electron uptake (EEU). While reductive-EET is long studied, EEU has come to fore recently, and represents a paradigm shift in microbial biogeochemistry. This is because EEU-capable microbes can oxidize abundant solid-phase conductive substances such as minerals for microbial growth. A number of microbes can perform EEU, but only a subset of these organisms can perform phototrophic-EEU. This specialized metabolism harnesses the energy of light and electrons from solid-conductive minerals or their proxies (poised electrodes) to fix carbon dioxide. Accordingly, phototrophic-EEU represents a new metabolism to engineer for sustainable, carbon-neutral to carbon-sequestering bioproduction. To understand the environmental prevalence of phototrophic-EEU, and realize its full bioengineering potential, several fundamental knowledge gaps must be addressed. These include in-depth characterization of the electron input modules and their regulation; and the electron transfer pathways and the cellular electron sinks. Using multi-omic and transdisciplinary approaches, this project aims to fill these knowledge gaps to gain a systems-level understanding of phototrophic-EEU in the freshwater microbe, Rhodopseudomonas palustris and the marine microbe Rhodovulum sulfidophilum. The project will use synthetic biology, metabolic engineering, and material science to improve sustainable production of bioplastics and biofuels using phototrophic-EEU. Together, this project will expand our understanding of phototrophic-EEU, and explore the production of sustainable bioproducts using the phototrophic-EEU capable microbe, Rhodopseudomonas palustris. This project will also engage students from various educational levels in sustainability research.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

一些细菌能够利用电力和光能来固定二氧化碳，并最终用于可再生的、碳中性的碳螯合生产某些高价值的化合物。 该项目的总体目标是更好地了解这一生物生产过程，并设计细菌将这一过程用于生物塑料和生物燃料生产。该项目将为高中，本科和研究生院的学生创造身临其境的研究机会。微生物的还原-氧化反应驱动生物圈中的物质和能量流动。大多数微生物使用可溶性电子供体和受体，但有些微生物使用固相导电物质。潜在的微生物过程被称为细胞外电子转移（EET）。EET期间电子流的方向性可以是出站，其中固相导电物质通过还原性EET被微生物还原;或入站，其中固相导电物质通过细胞外电子摄取（EEU）被氧化。虽然还原性EET已被长期研究，但EEU最近才崭露头角，代表了微生物生物地球化学的范式转变。这是因为具有EEU能力的微生物可以氧化大量的固相导电物质，如矿物质，以供微生物生长。许多微生物可以执行EEU，但只有这些生物体的一个子集可以执行光营养EEU。这种特殊的新陈代谢利用来自固体导电矿物或其代理物（平衡电极）的光能和电子来固定二氧化碳。因此，光营养EEU代表了一种新的代谢工程可持续的，碳中性的碳螯合生物生产。为了了解光营养EEU的环境流行，并实现其全部生物工程潜力，必须解决几个基本的知识差距。这些包括电子输入模块及其调节的深入表征;以及电子传递途径和细胞电子汇。利用多组学和跨学科的方法，该项目旨在填补这些知识空白，以获得淡水微生物，沼泽红球藻和海洋微生物Rhodovulum sulfidophilum中的光营养EEU的系统水平的了解。该项目将利用合成生物学、代谢工程和材料科学，利用光营养EEU改善生物塑料和生物燃料的可持续生产。总之，该项目将扩大我们对光营养EEU的理解，并探索使用光营养EEU能力微生物Rhodoptera palustris生产可持续生物产品。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Sustainable n-butanol production by a metabolically versatile anoxygenic phototrophic bacterium

代谢多功能的缺氧光养细菌可持续生产正丁醇

• 发表时间: 2020
• 期刊: bioRxiv
• 作者: Bai W, Ranaivoarisoa TO

Revised Draft Genome Sequences of Rhodomicrobium vannielii ATCC 17100 and Rhodomicrobium udaipurense JA643.

• DOI: 10.1128/mra.00022-21
• 发表时间: 2021-04-01
• 期刊: Microbiology resource announcements
• 影响因子: 0.8
• 作者: Conners EM;Davenport EJ;Bose A
• 通讯作者: Bose A

Harnessing Microbes to Produce Sustainable Plastics and Biofuels

利用微生物生产可持续塑料和生物燃料

• DOI: 10.33548/scientia730
• 发表时间: 2021
• 期刊: Scientia
• 作者: Bose, Arpita

Magnetite nanoparticle anchored graphene cathode enhances microbial electrosynthesis of polyhydroxybutyrate by Rhodopseudomonas palustris TIE-1

• DOI: 10.1088/1361-6528/abbe58
• 发表时间: 2020-10
• 期刊: Nanotechnology
• 影响因子: 3.5
• 作者: K. Rengasamy;T. Ranaivoarisoa;W. Bai;A. Bose

Improving bioplastic production by Rhodopseudomonas palustris TIE-1 using synthetic biology and metabolic engineering

• DOI: 10.1101/2023.05.17.541174
• 发表时间: 2023-05
• 期刊: bioRxiv
• 作者: T. Ranaivoarisoa;W. Bai;K. Rengasamy;Hope Steele;Miriam Silberman;Jennifer Olabode;A. Bose