Collaborative Research: Magnetically Assisted Self-Assembly for Facile 2D Membrane Protein Crystallization

合作研究:磁力辅助自组装轻松实现二维膜蛋白结晶

基本信息

  • 批准号:
    2023833
  • 负责人:
  • 金额:
    $ 27.76万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-09-01 至 2022-05-31
  • 项目状态:
    已结题

项目摘要

Non-Technical Membrane proteins are cells' primary mechanism for interacting with the external environment, and thus demonstrate exceptionally fine-tuned capabilities in sensing, selective transport, and catalysis. This award to study Magnetically Assisted Self-Assembly for Facile 2D Membrane Protein Crystallization will support fundamental research on the magnetic properties of these membrane proteins, and biological materials more generally. The PIs will leverage their knowledge of membrane protein behavior in magnetic fields to support the development of two-dimensional crystals from these biological materials. Finally, these crystals will be deployed in devices for highly selective sensing and separation applications. In parallel, the project will fund the expansion of an undergraduate research training and mentorship program that includes a formal 3-hour training process for PhD student supervisors of undergraduate students, a formal goal-setting process for incoming undergraduate researchers, and a 360-degree evaluation process for undergraduates, PhD supervisors, and the PI conducted on a bi-monthly basis and upon completion of the project.Technical PIs propose to investigate the fundamental physics and chemistry of membrane protein (MP) self-assembly in the presence of a magnetic field to inform strategies for scalable 2D crystallization of MPs with high crystalline order realized over large areas. They will compute the diamagnetic susceptibility of MPs and supporting matrices (block co-polymers and lipids) to assess the generalizability of this technique across known MPs and to inform subsequent self-assembly simulations. A novel coarse-grain model will be developed to describe the self-assembly of MP crystals in the presence and absence of an applied magnetic field. This model will be sufficiently efficient as to serve as a front-end biomaterial fabrication design tool across diverse combinations of MPs and supporting matrices. PIs will validate these models by experimentally characterizing the effect of magnetic fields on MP crystallization under diverse experimental conditions. Finally, 2D MP crystals of OmpF and pHR will be tested as model systems for screening ligands and blockers in comparison with current state-of-the-art bilayer type systems. This work will contribute 1) a novel approach for computing the diamagnetic anisotropy and diamagnetic susceptibility of large molecules in which primary, secondary, and tertiary structure each contribute to the magnetic properties of the molecule, 2) a physical understanding of the various competing forces that drive the kinetics and final state of self-assembly, 3) a modeling tool to guide experimental design for the fabrication of 2D MP crystals, and 4) a demonstration of 2D MP crystals in functional devices and an assessment of their performance relative to the current state-of-the-art.
非技术膜蛋白是细胞与外部环境相互作用的主要机制,因此在传感、选择性运输和催化方面表现出异常精细的能力。该奖项旨在研究易溶二维膜蛋白结晶的磁辅助自组装,将支持对这些膜蛋白和更广泛的生物材料的磁性的基础研究。pi将利用他们在磁场中膜蛋白行为的知识来支持这些生物材料的二维晶体的开发。最后,这些晶体将用于高选择性传感和分离应用的器件中。与此同时,该项目将资助扩大本科生研究培训和指导计划,其中包括对本科生博士生导师的正式3小时培训过程,对即将进入的本科生研究人员的正式目标设定过程,以及对本科生,博士生导师和PI的360度评估过程,每两个月进行一次,并在项目完成后进行。技术pi建议研究膜蛋白(MP)在磁场存在下自组装的基本物理和化学,为在大面积上实现高结晶顺序的MP的可扩展二维结晶策略提供信息。他们将计算MPs和支撑基质(嵌段共聚物和脂质)的抗磁化率,以评估该技术在已知MPs中的普遍性,并为随后的自组装模拟提供信息。一个新的粗粒模型将被开发来描述MP晶体的自组装在存在和缺乏外加磁场。该模型将足够有效,可以作为前端生物材料制造设计工具,跨越MPs和支持矩阵的不同组合。pi将在不同的实验条件下通过实验表征磁场对MP结晶的影响来验证这些模型。最后,将测试OmpF和pHR的二维MP晶体作为筛选配体和阻滞剂的模型系统,并与当前最先进的双层系统进行比较。这项工作将有助于1)计算大分子的抗磁各向异性和抗磁磁化率的新方法,其中一级,二级和三级结构每个都有助于分子的磁性,2)对驱动动力学和自组装最终状态的各种竞争力的物理理解,3)一个建模工具来指导2D MP晶体制造的实验设计。4)在功能器件中展示2D MP晶体,并评估其相对于当前最先进技术的性能。

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Competing Ion Behavior in Direct Electrochemical Selenite Reduction
Inadequacy of current approaches for characterizing membrane transport properties at high salinities
当前表征高盐度下膜传输特性的方法的不足
  • DOI:
    10.1016/j.memsci.2022.121246
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    9.5
  • 作者:
    Liang, Yuanzhe;Dudchenko, Alexander V.;Mauter, Meagan S.
  • 通讯作者:
    Mauter, Meagan S.
Direct Electrochemical Pathways for Selenium Reduction in Aqueous Solutions
Magnetic Field-Induced Alignment of Nanofibrous Supramolecular Membranes: A Molecular Design Approach to Create Tissue-like Biomaterials
磁场诱导的纳米纤维超分子膜排列:一种创建组织样生物材料的分子设计方法
  • DOI:
    10.1021/acsami.0c05191
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    9.5
  • 作者:
    Radvar, Elham;Shi, Yejiao;Grasso, Salvatore;Edwards-Gayle, Charlotte J.;Liu, Xitong;Mauter, Meagan S.;Castelletto, Valeria;Hamley, Ian W.;Reece, Michael J.;S. Azevedo, Helena
  • 通讯作者:
    S. Azevedo, Helena
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Meagan Mauter其他文献

Meagan Mauter的其他文献

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{{ truncateString('Meagan Mauter', 18)}}的其他基金

SusChEM: Collaborative Research: Identification of the critical length scales and chemistries responsible for the anti-fouling properties of heterogeneous surfaces
SusChEM:合作研究:确定负责异质表面防污性能的临界长度尺度和化学成分
  • 批准号:
    2023847
  • 财政年份:
    2019
  • 资助金额:
    $ 27.76万
  • 项目类别:
    Standard Grant
CAREER: INTEGRATED WATER, ENERGY, AND EMISSIONS DECISION MAKING FOR A LOW CARBON FUTURE WITH COAL-FIRED POWER PLANTS
职业:通过燃煤电厂实现水资源、能源和排放的综合决策,实现低碳未来
  • 批准号:
    2023841
  • 财政年份:
    2019
  • 资助金额:
    $ 27.76万
  • 项目类别:
    Standard Grant
Collaborative Research: INFEWS: N/P/H2O: Remote and autonomous sensing for managing the economic and environmental consequences of salinity-impacted agricultural waterways
合作研究:INFEWS:N/P/H2O:用于管理受盐度影响的农业水道的经济和环境后果的远程和自主传感
  • 批准号:
    2024004
  • 财政年份:
    2019
  • 资助金额:
    $ 27.76万
  • 项目类别:
    Standard Grant
Collaborative Research: Magnetically Assisted Self-Assembly for Facile 2D Membrane Protein Crystallization
合作研究:磁力辅助自组装轻松实现二维膜蛋白结晶
  • 批准号:
    1710143
  • 财政年份:
    2017
  • 资助金额:
    $ 27.76万
  • 项目类别:
    Continuing Grant
CAREER: INTEGRATED WATER, ENERGY, AND EMISSIONS DECISION MAKING FOR A LOW CARBON FUTURE WITH COAL-FIRED POWER PLANTS
职业:通过燃煤电厂实现水资源、能源和排放的综合决策,实现低碳未来
  • 批准号:
    1554117
  • 财政年份:
    2016
  • 资助金额:
    $ 27.76万
  • 项目类别:
    Standard Grant
Collaborative Research: INFEWS: N/P/H2O: Remote and autonomous sensing for managing the economic and environmental consequences of salinity-impacted agricultural waterways
合作研究:INFEWS:N/P/H2O:用于管理受盐度影响的农业水道的经济和环境后果的远程和自主传感
  • 批准号:
    1604853
  • 财政年份:
    2016
  • 资助金额:
    $ 27.76万
  • 项目类别:
    Standard Grant
SusChEM: Collaborative Research: Identification of the critical length scales and chemistries responsible for the anti-fouling properties of heterogeneous surfaces
SusChEM:合作研究:确定负责异质表面防污性能的临界长度尺度和化学成分
  • 批准号:
    1507850
  • 财政年份:
    2015
  • 资助金额:
    $ 27.76万
  • 项目类别:
    Standard Grant
Pseudocapacitive and Intercalation Compounds for Water Desalination: Surface Chemistry, Electrode Structure and Foulant Tolerance
用于海水淡化的赝电容和插层化合物:表面化学、电极结构和耐污性
  • 批准号:
    1403826
  • 财政年份:
    2014
  • 资助金额:
    $ 27.76万
  • 项目类别:
    Continuing Grant
SEES Fellows: Enabling Energy Efficiency through Integrated Utilities - Technical and Social Challenges to Forward Osmosis Microbial Bioreactors
SEES 研究员:通过综合公用事业提高能源效率 - 推进渗透微生物生物反应器的技术和社会挑战
  • 批准号:
    1215845
  • 财政年份:
    2012
  • 资助金额:
    $ 27.76万
  • 项目类别:
    Standard Grant

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