Quantitative proteomics to characterize the telosome

定量蛋白质组学来表征端粒

• 批准号: 243914686
• 负责人: Dr. Falk Butter
• 金额: --
• 依托单位: Institut für molekulare Virologie und Zellbiologie (IMVZ)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/243914686?language=en
• 关键词: Quantitative; proteomics; characterize; telosome

Telomeres as cap of most eukaryotic organisms consist of repetitivearrays bound by proteins to protect them from the DNA damageresponse (end protection problem) and are extended by a specificenzyme called telomerase to counteract their shortening (endreplication problem). While telomere maintenance is of paramountimportance in cell development, cancer and ageing, our knowledge ofthe key players and mechanistic understanding of this process is notcomplete. We here aim to identify and characterize previouslyunknown (direct) telomere binding proteins in diverse species. Usingstate-of-the-art quantitative mass spectrometry, we identify candidatesable to bind to the telomeric sequence and then combine this withmolecular biology, biochemistry and related assays to gain insight intothe protein functionality to provide a more comprehensive picture ofthe telosome.

端粒作为大多数真核生物的帽子，由蛋白质结合的重复阵列组成，以保护它们免受DNA损伤反应（末端保护问题），并由一种称为端粒酶的特定酶延长以抵消它们的缩短（末端复制问题）。虽然端粒维持在细胞发育、癌症和衰老中至关重要，但我们对关键参与者的认识和对这一过程的机制理解尚不完整。我们在这里的目的是鉴定和表征以前未知的（直接）端粒结合蛋白在不同的物种。使用最先进的定量质谱法，我们确定了可与端粒序列结合的候选者，然后将其与分子生物学，生物化学和相关分析相结合，以深入了解蛋白质功能，从而提供更全面的端粒图。