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Evaluation of a novel approach for in situ soft tissue engineering by external expansion

外部扩张原位软组织工程新方法的评估

基本信息

批准号：
247933787
负责人：
Professor Dr. Jürgen Bernhagen
金额：
--
依托单位：
Klinik für Plastische Chirurgie, Hand- und Verbrennungschirurgie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2013
资助国家：
德国
起止时间：
2012-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/247933787?language=en
关键词：
Evaluation novel approach situ soft

项目摘要

An adequate reconstruction of soft tissue defects is still an unsolved problem in reconstructive surgery. Adipose tissue engineering offers a potential solution but in vitro strategies are still afflicted with severe limitations. External soft tissue expansion by vacuum application is a new and promising approach for in situ adipose tissue engineering but little is known about the underlying mechanisms that lead to tissue augmentation. Here, we will apply vacuum as well as mechanical stress to primary human preadipocytes, endothelial cells and adipose tissue samples and will analyse the outcome with regard to potential mechanisms like inflammation, oxidative stress, and angiogenesis, all being crucial factors in adipogenesis. In particular, the levels of a panel of relevant secreted growth factors and cytokines including emerging adipogenic factors such as macrophage migration inhibitory factor (MIF) will be determined. The investigations will also encompass a global gene expression analysis and the evaluation of the phosphorylation state of regulatory proteins to identify responsible signaling pathways. The obtained in vitro results will be verified in an in vivo model using hairless rats, which mimics the external tissue expansion approach. The proposed project will significantly contribute to progress in the field of in situ tissue engineering and also will help to understand how obesity-related pathologies are associated with processes such as inflammation and oxidative stress.

软组织缺损的充分重建一直是软组织重建手术中尚未解决的问题。脂肪组织工程提供了一个潜在的解决方案，但体外策略仍然受到严重限制。体外真空软组织扩张是原位脂肪组织工程的一种很有前途的新方法，但对组织扩张的潜在机制知之甚少。在这里，我们将对原代人前脂肪细胞、内皮细胞和脂肪组织样本施加真空和机械应力，并分析其潜在机制的结果，如炎症、氧化应激和血管生成，这些都是脂肪形成的关键因素。特别是，一组相关的分泌生长因子和细胞因子的水平将被确定，包括新出现的脂肪生成因子，如巨噬细胞迁移抑制因子（MIF）。研究还将包括全球基因表达分析和调节蛋白磷酸化状态的评估，以确定负责的信号通路。获得的体外结果将在无毛大鼠体内模型中得到验证，该模型模仿外部组织扩张方法。该项目将对原位组织工程领域的进展做出重大贡献，也将有助于了解肥胖相关病理与炎症和氧化应激等过程的关系。