The MIF protein family in cardiac ischemia and heart failure: molecular mechanisms and translational avenues

心肌缺血和心力衰竭中的 MIF 蛋白家族：分子机制和转化途径

• 批准号: 443500595
• 负责人: Professor Dr. Jürgen Bernhagen
• 金额: --
• 依托单位: Klinik und Poliklinik für Anästhesiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/443500595?language=en
• 关键词: MIF; protein; family; cardiac; ischemia

Macrophage migration-inhibitory factor (MIF) is a structurally unique, inflammatory cytokine with chemokine-like activities that acts as a key upstream mediator in innate immunity and inflammation in the human body. It is the founding member of the MIF protein family, which encompasses D-dopachrome tautomerase (D-DT)/MIF-2 as well as a group of bacterial tautomerases and MIF orthologs across kingdoms. Through its unique expression characteristics such as semi- constitutive expression and rapid release upon inflammatory stimulation in immune cells, endothelial cells and cardiomyocytes, MIF is ideally positioned to serve as an early inflammatory and cell- stress sensor. Owing to this property and its inflammatory capacity, MIF was found to be a pivotal mediator in acute and chronic inflammatory and autoimmune diseases, such as septic shock and atherosclerosis. Although MIF activities in cardiovascular disease were initially defined in the context of atherosclerosis, work by us and others in the past 4-6 years has now established that MIF’s role in cardiovascular disease is complex and involves a fundamental role in the response to ischemic stress. In the heart, MIF is released by ischemic cardiomyocytes, fibroblasts, or endothelial cells and acts by auto-/paracrine signaling. Studies from our group and others have identified a unique role for MIF as a local cardiac cytokine with phase- specific cardioprotective and exacerbating activities. Our published work in the first funding period demonstrated that while increased MIF levels during myocardial ischemia/reperfusion were associated with a reduced risk for the development of organ dysfunctions, high MIF-2/D- DT levels were predictive of the development of atrial fibrillation and pneumonia, suggesting distinct functions of the MIF homologs in myocardial ischemia/reperfusion injury. Our own translational findings, and data obtained from recent meta-analysis, as well as the overall consensus of actual expert teams, led us to shift our focus from RIPC questions more towards an in-depth mechanistic understanding of the organ-protective effect of the perioperative increase in MIF and towards the following adaptation processes in the heart after myocardial ischemia/reperfusion, which frequently lead to the development of morbidities that are associated with significant healthcare-related costs.

巨噬细胞移动抑制因子(MIF)是一种结构独特的炎性细胞因子，具有趋化素样活性，是人体天然免疫和炎症的关键上游调节因子。它是MIF蛋白家族的创始成员，该家族包括D-DOPAChrome互换擦除酶(D-DT)/MIF-2以及一组跨王国的细菌互换擦除酶和MIF同源蛋白。由于其独特的表达特性，如免疫细胞、内皮细胞和心肌细胞在炎症刺激下的半结构性表达和快速释放，MIF被认为是一种理想的早期炎症和细胞应激传感器。由于MIF的这种特性和炎症能力，MIF被发现是感染性休克和动脉粥样硬化等急、慢性炎症性和自身免疫性疾病的关键介质。虽然MIF在心血管疾病中的活性最初是在动脉粥样硬化的背景下定义的，但我们和其他人在过去4-6年的工作中已经证实，MIF在心血管疾病中的作用是复杂的，涉及到对缺血应激反应的基础作用。在心脏，MIF由缺血心肌细胞、成纤维细胞或内皮细胞释放，并通过自身/旁分泌信号起作用。我们小组和其他人的研究已经确定了MIF作为一种局部心脏细胞因子的独特作用，具有时相特异性的心脏保护和加重活动。我们在第一个资助期发表的工作表明，虽然心肌缺血/再灌注期间MIF水平的增加与器官功能障碍的发生风险降低有关，但MIF-2/D-dt水平的高水平预示着心房颤动和肺炎的发生，这表明MIF同系物在心肌缺血/再灌注损伤中具有不同的功能。我们自己的翻译发现，以及从最近的荟萃分析中获得的数据，以及实际专家团队的总体共识，导致我们将重点从RIPC问题更多地转向对围手术期MIF增加的器官保护效应的深入机制理解，以及心肌缺血/再灌注后心脏的以下适应过程，这些过程经常导致与重大医疗保健相关成本相关的疾病的发生。