Sensor-Actor Systems to Combat Oral Peri-Implant Infections

对抗口腔种植体周围感染的传感器-作用系统

• 批准号: 254381848
• 负责人: Professor Dr. Henning Menzel
• 金额: --
• 依托单位: Institut für Technische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/254381848?language=en
• 关键词: Sensor; Actor; Systems; Combat; Oral

Dental implants exhibit a high prevalence of bacterial infections. These lead to inflammation, accompanied by tissue destruction, which can have serious consequences for the patient. During the research project novel implant systems are to be developed that permit specific release of antibacterial active substances (so-called effectors) as a reaction to the initial phase of infection. Thus, the release of the antibacterial effectors will be coupled to the infection or colonization, both spatially and temporally. During this project sensor-actor systems are to be developed that detect the signals by a sensor and activate an actor (signal conversion) through a trigger. The actor then releases effectors and thus initiates intervention. In order to implement these developments, the first step is to develop synthetic CELLULAR SENSOR-ACTOR SYSTEMS. With these systems the issues of principle can be examined related to the requirements for effective sensor systems and their conversion or possibly amplification in vitro and in the mouse model. Moreover, these cellular sensor systems can be used to amplify the signals and be coupled to ACELLULAR (CHEMICAL) ACTOR SYSTEMS. The first phase of the project will include quantitative studies on the necessary concentrations of signals and effectors, as well as the spatial conditions. This is to lead to the development of prototypes that demonstrate the feasibility in principle of these strategies in the mouse model. The insights won are to smooth the way to the development of acellular sensor-actor systems, in which chemical sensors are coupled to chemical effector systems. In the long term, the biomarkers and anti-biofilm active substances identified in the project ANALYSIS OF PATHOPHYSIOLOGY OF ORAL MULTI-SPECIES BIOFILMS AND IDENTIFICATION / CHARACTERISATION OF NOVAL ANTI-BIOFILM SUBSTANCES are to be used as signals or effectors for the developed sensor-actor systems. The pioneering basic research in synthetic biology on the production of cellular sensors, the applied polymer chemistry for the development of acellular sensors and the biofilm research are not only of importance for the immediate area of research. It is more the case that the integration of this knowledge on the development of implant-coupled sensor-effector systems may lead to a consistent improvement in the long-term prognosis for dental implants. Aside from the resulting long-term reduction in morbidity and costs for dental treatment, the project could make an essential contribution to the development of alternative strategies for the prevention, diagnosis and therapy of implant-associated infection, even in other areas of medicine.

牙科植入物表现出细菌感染的高患病率。这些导致炎症，伴随着组织破坏，这可能对患者造成严重后果。在该研究项目期间，将开发新型植入系统，该系统允许特异性释放抗菌活性物质（所谓的效应物）作为对感染初始阶段的反应。因此，抗菌效应物的释放将在空间上和时间上与感染或定殖偶联。在该项目中，将开发传感器-执行器系统，通过传感器检测信号并通过触发器激活执行器（信号转换）。然后，施动者释放效应器，从而启动干预。为了实现这些发展，第一步是开发合成的细胞传感器-作用系统。通过这些系统，可以检查与有效传感器系统的要求及其在体外和小鼠模型中的转换或可能的放大相关的原理问题。此外，这些细胞传感器系统可用于放大信号并与无细胞（化学）因子系统偶联。该项目的第一阶段将包括对信号和效应物的必要浓度以及空间条件进行定量研究。这将导致原型的开发，证明这些策略在小鼠模型中原则上的可行性。获得的见解是为非细胞传感器-作用器系统的发展铺平道路，在该系统中，化学传感器与化学效应器系统耦合。从长远来看，在口腔多物种生物膜病理生理学分析和新型抗生物膜物质的鉴定/纯化项目中鉴定的生物标志物和抗生物膜活性物质将用作开发的传感器-执行器系统的信号或效应器。在合成生物学中关于细胞传感器生产的开创性基础研究，用于非细胞传感器开发的应用聚合物化学和生物膜研究不仅对直接研究领域具有重要意义。更多的情况是，将这些知识整合到种植体耦合传感器效应器系统的开发中，可能会导致牙科种植体长期预后的持续改善。除了长期降低牙科治疗的发病率和成本外，该项目还可以为预防、诊断和治疗种植体相关感染的替代战略的发展做出重要贡献，甚至在其他医学领域。

项目成果

Session 14: Poster.

第 14 节：海报

• DOI: 10.1515/bmt-2019-7014
• 发表时间: 2019
• 期刊: Biomedizinische Technik. Biomedical engineering
• 作者: Bourgat Y;Mikolai C;Klahn P;Menzel H
• 通讯作者: Menzel H

Session 10: Biofilms Implant related infections

第 10 节：生物膜植入物相关感染

• DOI: 10.1515/bmt-2019-7010
• 发表时间: 2019
• 期刊: Biomedical Engineering / Biomedizinische Technik
• 作者: Mikolai C;Ingendoh-Tsakmakidis A;Winkel A;Kommerein N;Falk CS;Rossi A;Walles H;Stiesch M
• 通讯作者: Stiesch M