Verticillium-induced developmental reprogramming and resistance in Arabidopsis

拟开展的研究将集中在轮枝菌属维管真菌病原菌与模式植物拟南芥的相互作用。在初步工作中，我们发现轮枝菌分离物特异性建立无菌相互作用或诱导两种不同的发育重编程模式，其影响植物维管组织发育或腋生分生组织活性。计划研究的主要目标是在进化背景下识别和理解触发植物症状发展的谱系特异性轮枝菌效应分子的生物学作用和作用模式。此外，我们的目标是确定相应的植物蛋白质的目标和潜在的植物信号和执行机制，在拟南芥中负责复杂的相互作用表型。为此，我们将利用比较真菌基因组测序和最近开发的多亲本高级世代间杂交（MAGIC）拟南芥品系的表型可塑性在两个相互作用的合作伙伴的分子分析。总之，这项工作将有助于了解互惠选择如何塑造真菌病原体和它们的宿主植物之间的相互作用。

The proposed research will focus on the interaction of vascular fungal pathogens of the genus Verticillium with the model plant Arabidopsis. In preliminary work we found Verticillium isolate-specific establishment of symptomless interactions or induction of two distinct developmental reprogramming patterns that either affect plant vascular tissue development or axillary meristem activity. The major goal of the planned research is to identify and understand in an evolutionary context the biological role(s) and mode(s) of action of lineage-specific Verticillium effector molecules that trigger plant symptom development. Moreover, we aim at identifying corresponding plant protein targets and the underlying plant signaling and executive machineries in Arabidopsis that are responsible for the complex interaction phenotypes. To this end, we will utilize comparative fungal genome sequencing and recently developed Multiparent Advanced Generation Inter-cross (MAGIC) Arabidopsis lines for molecular analysis of phenotypic plasticity in both interaction partners. Together, this work will help to understand how reciprocal selection shapes the interactions between fungal pathogens and their host plants.