Functional analysis of Arabidopsis LysM-RLKs and related kinases in chitin perception and defense signaling

拟南芥 LysM-RLK 及相关激酶在几丁质感知和防御信号传导中的功能分析

• 批准号: 214362899
• 负责人: Professor Dr. Volker Lipka
• 金额: --
• 依托单位: Abteilung Zellbiologie der Pflanze
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/214362899?language=en
• 关键词: Functional; analysis; Arabidopsis; LysM; RLKs

In plants, many key processes such as development, reproduction, hormone signaling and defense against pathogens are regulated by receptor-like kinases (RLKs). It is becoming increasingly evident that plant transmembrane receptors are highly complex heteromers, consisting of a number of different RLKs as well as receptor-like cytoplasmic kinases (RLCKs). In the model plant Arabidopsis thaliana, the plasma-membrane localised LysM-RLK CERK1 mediates perception of the fungal PAMP chitin (and a yet unidentified bacterial danger signal) and subsequent activation of plant defence. This project focuses on the functional analysis of two other LysM-RLKs, LysM-RLK3 & 4, and a novel RLCK, CIR1, which we identified as potential heteromeric receptor complex partners of CERK1. We propose to analyse the role of LysM-RLK3, LysM-RLK4 and CIR1 in chitin signalling and other CERK1-mediated processes, to validate their interaction with CERK1 in planta and to study their subcellular localisation behaviour in response to elicitor treatment and pathogen attack. We expect novel mechanistic insights into PAMP-induced receptor complex formation, signalling and desensitization, as well as pathogen-induced cell polarization and dynamic protein trafficking that will extend our understanding of plant innate immunity.

在植物中，许多关键的过程，如发育、生殖、激素信号和防御病原体都受到受体样激酶（RLKs）的调节。越来越明显的是，植物跨膜受体是高度复杂的异聚体，由许多不同的RLK以及受体样胞质激酶（RLCK）组成。在模式植物拟南芥中，质膜定位的LysM-RLK CERK 1介导真菌PAMP几丁质（以及尚未鉴定的细菌危险信号）的感知和随后的植物防御激活。该项目的重点是对另外两个LysM-RLKs，LysM-RLK 3和4，以及一种新型RLCK，CIR 1的功能分析，我们将其确定为CERK 1的潜在异聚体受体复合物伴侣。我们建议分析LysM-RLK 3，LysM-RLK 4和CIR 1在几丁质信号传导和其他CERK 1介导的过程中的作用，以验证它们与CERK 1在植物中的相互作用，并研究它们在激发子处理和病原体攻击中的亚细胞定位行为。我们期待PAMP诱导的受体复合物的形成，信号传导和脱敏，以及病原体诱导的细胞极化和动态蛋白质运输，将扩大我们对植物先天免疫的理解新的机制的见解。