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Protein import into complex plastids

蛋白质输入复杂质体

基本信息

批准号：
263348573
负责人：
Professor Dr. Uwe Gallus Maier
金额：
--
依托单位：
Arbeitsgruppe Zellbiologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2014
资助国家：
德国
起止时间：
2013-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/263348573?language=en
关键词：
Protein import into complex plastids

项目摘要

Many algae evolved in secondary endosymbiosis. Here a unicellular, eukaryotic cell harboring a plastid surrounded by two membranes was internalized by another eukaryote. In the course of the co-evolution of this cell-within-a-cell the phototrophic cell was reduced to a complex plastid surrounded by either three or four membranes. From many genome projects it is known that the genomes of complex plastids have not the capacity to encode all plastid-located proteins. Thus, for the symbiontic interaction a transport system was established to guarantee transport of nucleus-encoded plastid protein across the plastid surrounding membranes. In case of the plastids of diatoms, which are surrounded by four membranes, we have identified for the first time the translocators of the second outermost and, in a cooperation project, of the third membrane. Additionally we have shown that these translocators are present in many other algae as well as in some intracellular parasites. In the project applied here, we will focus on the translocator complex in the second outermost membrane in respect to interacting factors and its structure. For that we will apply strategies firstly used in a secondarily evolved model organism. In addition it is planned to determine the protein structure of important translocator complex components. We expect from our work in-depth insights into the translocator complex and its functions. As this complex is in respect to its membrane components homologous to the ERAD machinery of eukaryotic cells, we expect that the community working on ERAD will profit from our work as well.

许多藻类是在次生内共生中进化的。在这里，一个单细胞，真核细胞含有一个被两层膜包围的叶绿体，被另一个真核生物内化。在这种细胞内细胞的共同进化过程中，光营养细胞被简化为被三层或四层膜包围的复杂的质体。从许多基因组计划中可以得知，复杂的质体基因组并不能编码所有的质体定位蛋白。因此，为了共生相互作用，建立了一个运输系统，以确保核编码的叶绿体蛋白通过叶绿体周围的膜进行运输。以被四层膜包围的硅藻为例，我们首次确定了第二层最外层的转运体，并在一个合作项目中确定了第三层膜的转运体。此外，我们已经证明，这些转运体存在于许多其他藻类以及一些细胞内寄生虫中。在这里应用的项目中，我们将重点研究第二最外层的膜上的转位复合体的相互作用因素及其结构。为此，我们将应用首先在二次进化的模式生物中使用的策略。此外，还计划确定重要转运体复合体成分的蛋白质结构。我们期待从我们的工作中深入了解移位器复合体及其功能。由于这个复合体的膜成分与真核细胞的ERAD机制同源，我们预计从事ERAD工作的社区也将从我们的工作中受益。