Integration of cell polarity, cell adhesion and actomyosin dynamics during epithelial morphogenesis

上皮形态发生过程中细胞极性、细胞粘附和肌动球蛋白动力学的整合

• 批准号: 273724513
• 负责人: Professor Dr. Stephan Wolfgang Grill
• 金额: --
• 依托单位: Max-Planck-Institut für molekulare Zellbiologie und Genetik (MPI-CBG)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/273724513?language=en
• 关键词: Integration; cell; polarity; adhesion; actomyosin

Epithelial morphogenesis in multicellular organisms depends on the coordination of cell polarity, cell adhesion and cell contractility. In this project we plan to decipher the genetic, cell biological and biophysical basis that underlies the regulation of actomyosin self-assembly and dynamics and its relation to junctional plasticity during dorsal closure in the Drosophila embryo. For this, we will make us of a novel fluctuation-based imaging technique (Co-moving Kinetic Analysis, COKA) to decipher the biochemical reaction network that gives rise to actomyosin cortical kinetics and dynamics. We will investigate the function of Crumbs, an apical regulator of epithelial polarity, on regulating actomyosin dynamics. Furthermore, we will investigate how the zonula adherens impacts actomyosin self-assembly and homeostasis, to identify the role that junctional components have in regulating actomyosin dynamics. Our work will identify how Crumbs, apico-basal cell polarity and the zoula adherens regulate dynamics of the actomyosin cytoskeletal dynamics to drive dorsal closure, an essential morphogenetic process in Drosophila development.

多细胞生物体中上皮的形态发生依赖于细胞极性、细胞粘附和细胞收缩性的协调。在这个项目中，我们计划破译的遗传，细胞生物学和生物物理学的基础上，调节肌动球蛋白的自组装和动力学及其与连接可塑性在果蝇胚胎的背关闭。为此，我们将使我们的一种新的波动为基础的成像技术（共同移动动力学分析，科卡）来破译的生化反应网络，引起肌动球蛋白皮质动力学和动力学。我们将研究Crumbs（一种上皮极性的顶端调节因子）在调节肌动球蛋白动力学方面的功能。此外，我们将研究如何影响粘连小带肌动球蛋白的自组装和稳态，以确定连接组件在调节肌动球蛋白动力学的作用。我们的工作将确定面包屑，apico-basal细胞极性和zoula adherens如何调节肌动球蛋白细胞骨架动力学的动态，以驱动背侧闭合，这是果蝇发育中一个重要的形态发生过程。