Integration of cell polarity, cell adhesion and actomyosin dynamics during epithelial morphogenesis

多细胞生物的上皮形态发生依赖于细胞极性、细胞粘附性和细胞收缩性的协调。在这个项目中，我们计划破译遗传，细胞生物学和生物物理基础，其背后的调节肌动球蛋白自组装和动力学及其与连接可塑性的关系在果蝇胚胎背关闭。为此，我们将采用一种新的基于波动的成像技术（共同运动动力学分析，COKA）来破译产生肌动球蛋白皮质动力学和动力学的生化反应网络。我们将研究面包屑的功能，上皮极性的顶端调节剂，在调节肌动球蛋白动力学。此外，我们将研究粘着带如何影响肌动球蛋白自组装和稳态，以确定连接成分在调节肌动球蛋白动力学中的作用。我们的工作将确定碎屑，顶基底细胞极性和zoula粘附体如何调节肌动球蛋白细胞骨架动力学的动力学，以驱动背闭合，这是果蝇发育中必不可少的形态发生过程。

Epithelial morphogenesis in multicellular organisms depends on the coordination of cell polarity, cell adhesion and cell contractility. In this project we plan to decipher the genetic, cell biological and biophysical basis that underlies the regulation of actomyosin self-assembly and dynamics and its relation to junctional plasticity during dorsal closure in the Drosophila embryo. For this, we will make us of a novel fluctuation-based imaging technique (Co-moving Kinetic Analysis, COKA) to decipher the biochemical reaction network that gives rise to actomyosin cortical kinetics and dynamics. We will investigate the function of Crumbs, an apical regulator of epithelial polarity, on regulating actomyosin dynamics. Furthermore, we will investigate how the zonula adherens impacts actomyosin self-assembly and homeostasis, to identify the role that junctional components have in regulating actomyosin dynamics. Our work will identify how Crumbs, apico-basal cell polarity and the zoula adherens regulate dynamics of the actomyosin cytoskeletal dynamics to drive dorsal closure, an essential morphogenetic process in Drosophila development.