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A quantitative analysis of forces and mechanics during interkinetic nuclear migration in the developing zebrafish embryo.

发育中斑马鱼胚胎运动核迁移过程中力和力学的定量分析。

基本信息

批准号：
245415555
负责人：
Professor Dr. Stephan Wolfgang Grill
金额：
--
依托单位：
Max-Planck-Institut für molekulare Zellbiologie und Genetik (MPI-CBG)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2013
资助国家：
德国
起止时间：
2012-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/245415555?language=en
关键词：
quantitative analysis forces mechanics during

项目摘要

Interkinetic Nuclear Migration (IKNM) describes cell cycle phase dependent nuclear movements in pseudostratified epithelia in a broad range of developing organisms from the anemone nematostella to the mammalian neocortex. Despite the fact that nuclear movements during IKNM are thought to be intimately tied to successful proliferation and subsequent differentiation, not much is known about the relationship between IKNM and developmental programs. Recently some progress has been made in understanding how distinct proteins, especially cytoskeletal elements and their motors, influence nuclear movement during IKNM on a single cell basis. What is still lacking however is an appreciation of the phenomenon in space and time taking all epithelial nuclei and other subcellular arrangements into consideration. We here aim to elicit nuclear movements beyond the single cell level in zebrafish neuroepithelia in vivo, developing new tracking algorithms that will permit to obtain a holistic picture of all nuclear trajectories. This dataset will help to understand how nuclear movements influence tissue architecture and whether the dynamics of nuclear movement in IKNM are cause or consequence for tight nuclear packing. We will further investigate the links between the tissue scale organization of IKNM and the intracellular forces that lead to it, mainly focusing on the actin and the microtubule cytoskeleton. The outcome of the experimental work will be a prerequisite for a theoretical approach describing IKNM as an emergent property of the tightly packed epithelium, which is the ultimate goal of this proposal.

间动力核迁移（IKNM）描述了从海葵线虫到哺乳动物新皮层的广泛发育生物体中假复层上皮细胞中细胞周期时相依赖的核运动。尽管IKNM过程中的核运动被认为与成功的增殖和随后的分化密切相关，但关于IKNM和发育程序之间的关系知之甚少。最近已经取得了一些进展，在了解不同的蛋白质，特别是细胞骨架元素和他们的马达，影响核运动IKNM在单细胞的基础上。然而，仍然缺乏的是考虑到所有上皮细胞核和其他亚细胞排列对空间和时间现象的理解。我们在这里的目标是引起核运动超出单细胞水平在斑马鱼神经上皮细胞在体内，开发新的跟踪算法，将允许获得所有核轨迹的整体图片。该数据集将有助于了解核运动如何影响组织结构，以及IKNM中核运动的动力学是否是紧密核包装的原因或后果。我们将进一步研究IKNM的组织规模组织和导致它的细胞内力量之间的联系，主要集中在肌动蛋白和微管细胞骨架。实验工作的结果将是一个先决条件的理论方法描述IKNM作为一个紧急的属性的紧密包装的上皮，这是本提案的最终目标。