Cause and consequence: the contribution of the host´s microtubule cytoskeleton to Chlamydia pneumoniae infection

许多微生物病原体靶向宿主细胞骨架以促进感染过程。严格的细胞内革兰氏阴性细菌病原体衣原体改变真核细胞骨架，以促进入侵，复制和传播。肌动蛋白细胞骨架在衣原体感染过程中的作用已被充分证明，但宿主微管细胞骨架的影响尚不清楚。在本项目中，我们将确定微管细胞骨架在人类病原体肺炎衣原体进入宿主细胞和发育周期中的作用。这种微生物以一种不确定的方式沿着沿着微管轨道移动到宿主细胞的微管组织中心，迄今为止只有一个C。肺炎杆菌蛋白CopN已被表征为体外微管调节剂。因此，我们将确定衣原体效应蛋白，调节微管和确定这些蛋白质的贡献衣原体感染周期。作为C.在异源性肺炎链球菌中鉴定和表征的微管调节蛋白在遗传上是不可接近的。粟酒裂殖酵母系统最后，我们将确定当宿主细胞的微管细胞骨架被C.肺炎。由于这种病原体的感染很常见，并且与肺癌风险增加有关，我们将分析细菌感染是否干扰有丝分裂过程，从而导致非整倍体，这是癌症的标志之一。

Many microbial pathogens target the host cytoskeleton to promote the infection process. The strictly intracellular gram-negative bacterial pathogens Chlamydiae alter the eukaryotic cytoskeleton to facilitate invasion, replication and dissemination. The role of the actin cytoskeleton during the chlamydial course of infection has been well documented however the impact of the host´s microtubule cytoskeleton is less clear. In this project we will determine the role of the microtubule cytoskeleton during host cell entry and the developmental cycle of the human pathogen Chlamydia pneumoniae. This organism travels along microtubule tracks to the host´s cell microtubule organizing center in a non-defined manner and to date only a single C. pneumoniae protein, CopN, has been characterized as an in vitro microtubule modulator. Thus we will identify chlamydial effector proteins that modulate microtubules and determine the contribution of such proteins to the chlamydial infection cycle. As C. pneumoniae is not genetically accessible bacterial microtubule regulating proteins will be identified and characterized in the heterologous S. pombe fission yeast system. Lastly, we will determine the consequences for the host cell when its microtubule cytoskeleton is subverted by C. pneumoniae. As infection by this pathogen is common and has been associated with an increased risk for lung cancer we will analyze if bacterial infection interferes with mitotic processes and thus leads to aneuploidy, one of the hallmarks of cancer.