Composition and metabolism of the pellicle in children

儿童表膜的组成和代谢

• 批准号: 276091689
• 负责人: Professor Dr. Christian Hannig
• 金额: --
• 依托单位: Professur für Kariologie, Zahnhartsubstanzlehre und Endodontie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/276091689?language=en
• 关键词: Composition; metabolism; pellicle; children

The inital bioadhesion on the tooth surface and pellicle formation have a key role for physiological and pathophysiological processes in the oral cavity. Composition and structure of the pellicle in adults have been investigated in detail. Though it is well known that oral environment and salivary composition in children and adults differ considerably, the pellicle in children has not been investigated adequately until now. The present project aims to evaluate proteome, metabolome and enzymology in the pellicle of preschool children systematically. Children with different caries activity will be enrolled in the study. In addition, the ultrastructure of the pellicle will be visualized by electron microscopy and fluorescence microscopy. The metabolome of the pellicle will be evaluated for the first time; this offers new insights in the metabolism at the tooth surface. Combined analysis of the respective parameters offers better and profound understanding of physiological and pathophysiological processes in the initial phase of in situ bioadhesion in the oral cavity.

牙表面的初始生物粘附和牙膜的形成在口腔的生理和病理生理过程中起着关键作用。对成虫的膜的组成和结构进行了详细的研究。虽然众所周知，儿童和成人的口腔环境和唾液成分有很大的不同，但到目前为止，儿童的口腔粘膜膜还没有得到充分的研究。本项目旨在系统地评价学龄前儿童细胞膜的蛋白质组、代谢组和酶学。有不同龋齿活动的儿童将被纳入研究。此外，将通过电子显微镜和荧光显微镜观察膜的超微结构。将首次对细胞膜代谢组进行评估；这为牙齿表面的新陈代谢提供了新的见解。综合分析各项参数，可以更好、更深入地了解口腔原位生物粘附初期的生理和病理生理过程。