Composition and metabolism of the pellicle in children

儿童表膜的组成和代谢

• 批准号: 276091689
• 负责人: Professor Dr. Christian Hannig
• 金额: --
• 依托单位: Professur für Kariologie, Zahnhartsubstanzlehre und Endodontie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/276091689?language=en
• 关键词: Composition; metabolism; pellicle; children

The inital bioadhesion on the tooth surface and pellicle formation have a key role for physiological and pathophysiological processes in the oral cavity. Composition and structure of the pellicle in adults have been investigated in detail. Though it is well known that oral environment and salivary composition in children and adults differ considerably, the pellicle in children has not been investigated adequately until now. The present project aims to evaluate proteome, metabolome and enzymology in the pellicle of preschool children systematically. Children with different caries activity will be enrolled in the study. In addition, the ultrastructure of the pellicle will be visualized by electron microscopy and fluorescence microscopy. The metabolome of the pellicle will be evaluated for the first time; this offers new insights in the metabolism at the tooth surface. Combined analysis of the respective parameters offers better and profound understanding of physiological and pathophysiological processes in the initial phase of in situ bioadhesion in the oral cavity.

牙齿表面和颗粒形成的易生粘附在口腔中的生理和病理生理过程中具有关键作用。成人中颗粒的组成和结构已详细研究。尽管众所周知，儿童和成人的口腔环境和唾液成分有很大差异，但迄今为止，儿童的颗粒尚未得到充分研究。本项目旨在系统地评估学龄前儿童颗粒中的蛋白质组，代谢组和酶学。该研究将招募不同的龋齿活动的儿童。另外，将通过电子显微镜和荧光显微镜观察到颗粒的超微结构。颗粒的代谢组将首次评估；这为牙齿表面的新陈代谢提供了新的见解。对各自参数的组合分析在口腔原位生物粘附的初始阶段中对生理和病理生理过程提供了更好的深刻理解。