Initial biofilm formation in caries-active and caries-inactive individuals

龋齿活跃和龋齿非活跃个体的初始生物膜形成

• 批准号: 224849956
• 负责人: Professor Dr. Christian Hannig
• 金额: --
• 依托单位: Professur für Kariologie, Zahnhartsubstanzlehre und Endodontie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/224849956?language=en
• 关键词: Initial; biofilm; formation; caries; active

Caries still is the infectious disease with highest prevalence worldwide and it is of considerable economic relevance. Initial bioadhesion and pellicle formation are essential for the establishment of a caries-pathogenic biofilm on the tooth surface. However, until now function and structure of the in situ pellicle and its initial bacterial colonization have not been evaluated so far with respect to caries activity and caries susceptibility. For the first time the pellicles of caries-active and caries inactive adult subjects will be characterized with respect to structure, function and initial bacterial colonization in the submitted project. For this purpose, enzymatic, electron-microscopic and fluorescence-microscopic techniques will be applied; the adherent bacteria will be evaluated with biomolecular techniques (DGGE, T-RFLP). In addition, the adherent S. mutans species will be explored with molecular mass spectrometry. Strong emphasis is laid on glucosyltransferases (GTF), their products (glucans) and their interactions with other pellicle components as well as with the adherent bacteria. GTFs are relevant key proteins for the process of biofilm formation, but up to now, the interactions of GTFs with other pellicle components have only been investigated in vitro. It is aimed to characterize the process of initial bioadhesion with respect to the individual caries activity in detail. The results will contribute to basic research a as well as to development of new strategies in caries prevention.

龋齿仍然是全世界患病率最高的传染病，它具有相当大的经济意义。初始生物粘附和颗粒形成对于在牙齿表面建立龋齿性生物膜是必不可少的。但是，到目前为止，迄今尚未在龋齿活动和龋齿敏感性方面评估原位颗粒及其初始细菌定植的功能和结构。在提交项目中，龋齿活性和龋齿不活跃的成年受试者的颗粒将首次以结构，功能和初始细菌定殖为特征。为此，将应用酶，电子显微镜和荧光 - 微观技术。将使用生物分子技术（DGGE，T-RFLP）评估粘附细菌。另外，将使用分子质谱法探索粘附的菌群。非常重视葡萄糖基转移酶（GTF），其产物（葡萄糖）及其与其他细胞成分以及与粘附细菌的相互作用。 GTF是生物膜形成过程的关键蛋白，但是直到现在，GTF与其他颗粒成分的相互作用仅在体外研究了。它的目的是详细介绍有关单个龋齿活动的初始生物粘附过程。结果将有助于基础研究以及预防龋齿的新策略的发展。