Identification and regulation of the stilbene biosynthetic pathway genes from grapevine

葡萄二苯乙烯生物合成途径基因的鉴定与调控

• 批准号: 278267466
• 负责人: Professor Dr. Jochen Bogs
• 金额: --
• 依托单位: Dienstleistungszentrum Ländlicher Raum Rheinpfalz
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/278267466?language=en
• 关键词: Identification; regulation; stilbene; biosynthetic; pathway

Plant stilbenes are phytoalexins that accumulate in a number of plant species in response to biotic and abiotic stresses. In recent years, stilbenes have received widespread attention in medical research because of its implication in many beneficial effects on human health. Stilbenes have been detected in at least 72 plant species including crops like peanut, blueberry and grapevine. Plants accumulate stilbenes in significant amounts not only in vegetative parts, but also in fruits, which are used as food or in the production of juices and wines. In contrast to the large diversity of described stilbenes with different biological activities, only two enzymes of the stilbene pathway have been identified and characterized up to now. Considering the various modifications of the basic stilbene skeleton and the transport of stilbenes additional unknown genes and gene products have to be present for these processes. Also the regulation of the stilbene pathway on the molecular level was unknown untill the recent identification and characterization of the two grapevine transcription factors VvMYB14 and VvMYB15 specifically regulating the stilbene pathway (Höll et al., 2013). Overexpression of these transcription factors and subsequent microarray, RNAseq and chromatin immunoprecipitation (ChiP) analysis will be used in the applied research project to initially identify the target genes of the transcription factors. Thereby, yet unknown genes of the stilbene pathway can be analysed and their regulation be studied in detail. Ectopic expression of these structural genes will allow the biochemical characterization of the respective gene products. Additionally, genetic differences of grapevine cultivars or species and influences of environmental factors on the stilbene pathway related to the optimization of grapevine stilbene content will be studied. Therefore, this research project will provide new insights into the molecular basis and the regulation of the stilbene pathway in plants, which is still poorly understood. The basic results concerning the genetic differences of grapevine cultivars or species and the impact of environmental factors on stilbene metabolism could allow optimisation of grapevine stilbene content via subsequent grapevine breeding approaches or adaptation of viticultural practices.

植物二苯乙烯是植物抗毒素，积累在许多植物物种响应生物和非生物胁迫。近年来，二苯乙烯因其对人体健康的诸多益处而受到医学研究的广泛关注。在花生、蓝莓和葡萄藤等至少72种植物中发现了芪类化合物。植物不仅在营养部分积累了大量的芪，而且在水果中也积累了大量的芪，这些水果被用作食物或生产果汁和葡萄酒。与已描述的具有不同生物活性的芪类化合物种类繁多相比，迄今为止仅鉴定和表征了两种芪类途径的酶。考虑到二苯乙烯基本骨架的各种修饰和二苯乙烯的运输，这些过程中必须存在额外的未知基因和基因产物。此外，在分子水平上对二苯乙烯途径的调控也是未知的，直到最近鉴定和表征了两个专门调节二苯乙烯途径的葡萄转录因子VvMYB14和VvMYB15 （Höll et al., 2013）。这些转录因子的过表达和随后的微阵列、RNAseq和染色质免疫沉淀（ChiP）分析将用于应用研究项目，以初步确定转录因子的靶基因。从而可以对芪烯通路的未知基因进行分析，并对其调控进行详细的研究。这些结构基因的异位表达将允许各自的基因产物的生化表征。此外，还将研究葡萄品种或品种的遗传差异以及环境因素对与葡萄芪含量优化相关的芪途径的影响。因此，本研究项目将对植物中尚不清楚的芪烯通路的分子基础和调控机制提供新的认识。葡萄品种遗传差异和环境因素对stilbene代谢的影响的基本结果可以通过后续的葡萄育种方法或适应葡萄栽培方法来优化葡萄stilbene含量。

项目成果

Combinatorial Regulation of Stilbene Synthase Genes by WRKY and MYB Transcription Factors in Grapevine (Vitis vinifera L.)

• DOI: 10.1093/pcp/pcy045
• 发表时间: 2018-05
• 期刊: Plant and Cell Physiology
• 影响因子: 4.9
• 作者: A. Vannozzi;D. Wong;Janine H[REPLACEMENT CHARACTER]ll;Ibrahim Hmmam;J. T. Matus;J. Bogs;Tobias Ziegler;I. Dry;G. Barcaccia;M. Lucchin