Improvement of the central microscopy research infrastructure Center for Advanced Imaging (CAi) at Heinrich Heine University Düsseldorf

杜塞尔多夫海因里希-海涅大学先进成像中心（CAi）是一个设备齐全的中央研究基础设施，提供最先进的成像和显微镜技术，可供内部和外部研究人员使用。在这个项目中，我们希望将基础设施进一步发展为三个方向。研究重点：一是鼓励和拓展CAi的研究重点，通过与分子物理化学研究所的密切合作，为多参数荧光成像光谱（MFIS）的应用提供了独特的技术和智力环境。在这个项目中，我们将开发结构，使这项技术的应用可用于更多的内部和外部研究小组。我们将为MFIS实验制定标准化的工作流程和协议，我们将为MFIS提供额外的培训和讲习班。由于我们配备了最新的超分辨率显微镜系统，我们将在CAi建立超分辨率显微镜的第二个研究重点。在这里，我们将重点介绍通过测试各种荧光团来建立和进一步发展不同超分辨率技术的染色方案。结果将在我们的网络中记录并发布，以便社区使用。研究数据管理：第二，我们将与ZIM海因里希-海涅大学计算中心密切合作，改进成像数据的数据处理。我们将制定存储研究数据的实用指南，这些数据可以转移到HHU的其他研究所和中央研究基础设施。我们还将在中央文件服务器上安装OME Remote Objects，以提供图像数据处理和存储的工具。在HHU的长期数据存储项目中，计算中心将探索使用OMERO作为长期存储成像数据平台的可能性。本地能力网络：在本地成像研讨会中，我们将汇集本地研究人员，应用先进的显微镜方法。此外，我们希望探讨在我们莱茵/鲁尔地区的成像设施之间建立官方网络的可能性。该讲习班将由CAi和科隆CECAD卓越集群的成像设施负责人组织。

The Center for advanced imaging (CAi) at Heinrich-Heine University of Duesseldorf is a well-equipped central research infrastructure that provides state of the art imaging and microscopy and is accessible for internal and external researchers. Within this project we want to further develop this infrastructure into three directions. Research focus fluorescence imaging spectroscopy: First, we want to encourage and extend the research focus of CAi: We provide a unique technical and intellectual environment for the application of multi-parameter fluorescence image spectroscopy (MFIS) due to close cooperation with the Institute for Molecular Physical Chemistry. Within this project we will develop structures to make the application of this technique available for more internal and external research groups. We will develop a standardized workflow and protocols for MFIS experiments and we will offer additional trainings and workshops for MFIS. As we are equipped with the latest super resolution microscopy systems we will establish a second research focus for super resolution microscopy at CAi. Here we will focus on the establishment and further development of staining protocols for the different super resolution techniques by testing various fluorophores. The results will be documented within our network and published to make them available for the community. Research data management: Second, we will improve the data handling of imaging data in close cooperation with the computing center at Heinrich-Heine University, ZIM. We will develop practical guidelines for storage of research data, which can be transferred to other institutes and central research infrastructures at HHU. We will furthermore install OME Remote Objects on the central file server to provide a tool for image data handling and storage. Within the project for long-term data storage at HHU the computing center will explore the possibility to use OMERO as platform for long-term storage of imaging data. Local competence network: Within a local imaging workshop we will bring together local researchers applying advanced microscopy methods. Furthermore we want to explore the possibilities to establish an official network between the imaging facilities of our region Rhine/Ruhr area. The workshop will be organized by CAi and the head of the imaging facility of the cluster of excellence CECAD in Cologne.