Improvement of the central microscopy research infrastructure Center for Advanced Imaging (CAi) at Heinrich Heine University Düsseldorf

杜塞尔多夫海因里希-海涅大学的先进成像中心（CAi）是一个设备齐全的中央研究基础设施，提供最先进的成像和显微镜，可供内部和外部研究人员使用。在这个项目中，我们希望进一步发展这个基础设施到三个方向。研究重点荧光成像光谱：首先，我们希望鼓励和扩展CAi的研究重点：由于与分子物理化学研究所的密切合作，我们为多参数荧光图像光谱（MFIS）的应用提供了独特的技术和知识环境。在这个项目中，我们将开发结构，使更多的内部和外部研究小组可以应用这项技术。我们将为MFIS实验制定标准化的工作流程和协议，并将为MFIS提供额外的培训和研讨会。由于我们配备了最新的超分辨率显微镜系统，我们将在CAi建立超分辨率显微镜的第二个研究重点。在这里，我们将集中在建立和进一步发展的染色协议，为不同的超分辨率技术，通过测试各种荧光团。结果将记录在我们的网络中，并公布于众。研究数据管理：其次，我们将与海因里希-海涅大学的计算中心ZIM密切合作，改善成像数据的数据处理。我们将制定研究数据存储的实用指南，这些数据可以转移到HHU的其他研究所和中央研究基础设施。此外，我们会在中央档案伺服器上安装OME Remote Objects，以提供一个处理及储存影像数据的工具。在HHU的长期数据存储项目中，计算中心将探索使用OMERO作为成像数据长期存储平台的可能性。当地能力网络：在当地的成像研讨会上，我们将汇集当地研究人员应用先进的显微镜方法。此外，我们希望探索在我们地区莱茵河/鲁尔地区的成像设施之间建立正式网络的可能性。该讲习班将由CAi和科隆的CECAD示范组成像设施负责人组织。

The Center for advanced imaging (CAi) at Heinrich-Heine University of Duesseldorf is a well-equipped central research infrastructure that provides state of the art imaging and microscopy and is accessible for internal and external researchers. Within this project we want to further develop this infrastructure into three directions. Research focus fluorescence imaging spectroscopy: First, we want to encourage and extend the research focus of CAi: We provide a unique technical and intellectual environment for the application of multi-parameter fluorescence image spectroscopy (MFIS) due to close cooperation with the Institute for Molecular Physical Chemistry. Within this project we will develop structures to make the application of this technique available for more internal and external research groups. We will develop a standardized workflow and protocols for MFIS experiments and we will offer additional trainings and workshops for MFIS. As we are equipped with the latest super resolution microscopy systems we will establish a second research focus for super resolution microscopy at CAi. Here we will focus on the establishment and further development of staining protocols for the different super resolution techniques by testing various fluorophores. The results will be documented within our network and published to make them available for the community. Research data management: Second, we will improve the data handling of imaging data in close cooperation with the computing center at Heinrich-Heine University, ZIM. We will develop practical guidelines for storage of research data, which can be transferred to other institutes and central research infrastructures at HHU. We will furthermore install OME Remote Objects on the central file server to provide a tool for image data handling and storage. Within the project for long-term data storage at HHU the computing center will explore the possibility to use OMERO as platform for long-term storage of imaging data. Local competence network: Within a local imaging workshop we will bring together local researchers applying advanced microscopy methods. Furthermore we want to explore the possibilities to establish an official network between the imaging facilities of our region Rhine/Ruhr area. The workshop will be organized by CAi and the head of the imaging facility of the cluster of excellence CECAD in Cologne.