Improvement of the central microscopy research infrastructure Center for Advanced Imaging (CAi) at Heinrich Heine University Düsseldorf

位于杜塞尔多夫的海因里希-海因大学的高级成像中心(CAI)是一个设备齐全的中央研究基础设施，提供最先进的成像和显微镜，内部和外部研究人员都可以访问。在这个项目中，我们希望将这个基础设施进一步发展为三个方向。研究重点：首先，我们希望鼓励和扩大CAI的研究重点：由于与分子物理化学研究所的密切合作，我们为多参数荧光图像光谱(MFIS)的应用提供了独特的技术和智力环境。在这个项目中，我们将开发结构，使更多的内部和外部研究小组可以应用这项技术。我们将为微型金融机构的实验制定标准化的工作流程和协议，并将为微型金融机构提供额外的培训和讲习班。由于我们配备了最新的超分辨率显微镜系统，我们将在中国工程院建立第二个超分辨率显微镜研究中心。在这里，我们将通过测试不同的荧光团，重点建立和进一步发展不同超分辨技术的染色方案。结果将在我们的网络中记录下来并发布，以供社区使用。研究数据管理：第二，我们将与海因里希-海因大学计算中心密切合作，完善成像数据的数据处理。我们将制定实用的研究数据存储指南，可将其转移到其他研究所和HHU的中央研究基础设施。我们还将在中央文件服务器上安装OME远程对象，为图像数据处理和存储提供工具。在HHU长期数据存储项目中，计算中心将探索使用OMERO作为长期存储成像数据的平台的可能性。本地能力网络：在一个本地成像研讨会中，我们将召集应用先进显微镜方法的本地研究人员。此外，我们希望探讨在我们区域莱茵河/鲁尔地区的成像设施之间建立官方网络的可能性。研讨会将由蔡和科隆卓越CECAD集群成像设施的负责人组织。

The Center for advanced imaging (CAi) at Heinrich-Heine University of Duesseldorf is a well-equipped central research infrastructure that provides state of the art imaging and microscopy and is accessible for internal and external researchers. Within this project we want to further develop this infrastructure into three directions. Research focus fluorescence imaging spectroscopy: First, we want to encourage and extend the research focus of CAi: We provide a unique technical and intellectual environment for the application of multi-parameter fluorescence image spectroscopy (MFIS) due to close cooperation with the Institute for Molecular Physical Chemistry. Within this project we will develop structures to make the application of this technique available for more internal and external research groups. We will develop a standardized workflow and protocols for MFIS experiments and we will offer additional trainings and workshops for MFIS. As we are equipped with the latest super resolution microscopy systems we will establish a second research focus for super resolution microscopy at CAi. Here we will focus on the establishment and further development of staining protocols for the different super resolution techniques by testing various fluorophores. The results will be documented within our network and published to make them available for the community. Research data management: Second, we will improve the data handling of imaging data in close cooperation with the computing center at Heinrich-Heine University, ZIM. We will develop practical guidelines for storage of research data, which can be transferred to other institutes and central research infrastructures at HHU. We will furthermore install OME Remote Objects on the central file server to provide a tool for image data handling and storage. Within the project for long-term data storage at HHU the computing center will explore the possibility to use OMERO as platform for long-term storage of imaging data. Local competence network: Within a local imaging workshop we will bring together local researchers applying advanced microscopy methods. Furthermore we want to explore the possibilities to establish an official network between the imaging facilities of our region Rhine/Ruhr area. The workshop will be organized by CAi and the head of the imaging facility of the cluster of excellence CECAD in Cologne.