ゲノム情報から読み解く薬剤耐性感染症におけるキャリアとしての伴侶動物の重要性

从基因组信息中解读伴侣动物作为耐药感染携带者的重要性

• 批准号: 20K10432
• 负责人: 嶋田 照雅
• 金额: $ 2.91万
• 依托单位: Osaka Metropolitan University (2022)Osaka Prefecture University (2020-2021)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K10432/
• 关键词: 薬剤耐性感染症; 伴侶動物; 伝播; 第三世代セファロスポリン耐性腸内細菌科細菌; 第３世代セファロスポリン耐性腸内細菌科細菌

薬剤耐性菌（第三世代セファロスポリン耐性腸内細菌科細菌：3GCREnt)の伝播において伴侶動物の役割と影響を明らかにすることを目的としている。本年度の成果は以下の通りである。新たにイヌの病巣または糞便由来3GCR大腸菌を5株取得した。院内環境における耐性菌の分布調査では、本年度は調査したいずれの箇所（のべ94箇所）においても3GCREntは検出されなかった。獣医療従事者を対象に行われたインフォームド・コンセントで同意が得られた研究協力者13名の糞便から13株の3GCREntが分離された。これら18株を対象に全ゲノムシーケンス解析を行った。イヌ由来3GCR大腸菌の生化学性状解析と全ゲノムシーケンス解析の結果、ESBL大腸菌の１株はmcr-1遺伝子をプラスミド上に保持し、コリスチン耐性を示すことが明らかとなった。イヌのコリスチン耐性ESBL大腸菌の報告は本邦初であり、国際学術誌に掲載された。今後動物・環境・ヒト由来の3GCREntの比較性状（生化学的ならびに遺伝学的）解析を行うことで異種間伝播に重要な菌株やプラスミドタイプが明らかになることが期待される。

The third generation of resistant bacteria (the third generation of bacteria in the family of endurance: 3GCREnt) was used to sow the bacteria with the animals cut in the service. The following are the achievements of this year. Five strains of 3GCR were obtained from the disease. The distribution of resistant bacteria in the environment of the hospital is very common, and this year, the laboratory (Institute 94) is responsible for the distribution of resistant bacteria in the hospital. This year, the number of bacteria in the hospital is very high, and the distribution of resistant bacteria in the environment is very common in the hospital. This year, the laboratory (Institute 94) is responsible for the distribution of bacteria in the hospital. A total of 13 3GCREnt strains were isolated from 13 people who agreed to cooperate in the study. Eighteen plants were isolated from the whole plant. Analysis of biochemical characteristics of 3GCR spp. the results of the analysis of the biochemical characteristics of the bacteria, the results of the analysis of the biochemical characteristics of the bacteria of 3GCR, the results of the analysis of the biochemical characteristics of the bacteria, the results of the analysis of the biochemical characteristics of the bacteria, the results of the analysis of the biochemical characteristics of the bacteria, the results of the analysis of the biochemical characteristics of the bacteria, the results of the analysis of the biochemical characteristics of the bacteria, the results of the analysis of the biochemical characteristics of the bacteria, the results of the analysis of the biochemical characteristics of the bacteria, the results of the analysis, the results of the analysis of the biochemical characteristics of the bacteria, the results of the analysis of the biochemical characteristics of the bacteria, the results of the analysis of the biochemical characteristics, the results of the analysis, the results of the analysis of the biochemical characteristics and the results of The report of ESBL-tolerant bacteria was reported at the beginning of the state and the journal of national studies. In the future, the origin of the animal environment has led to the analysis of 3GCREnt characteristics (from biochemical chemistry). It is necessary to analyze the important strains that are used to sow important strains among all kinds of animals.

项目成果

大阪府立大学生命環境科学域附属 獣医臨床センター

大阪府立大学生命环境科学部兽医临床中心

大阪公立大学獣医学部附属獣医臨床センター

大阪公立大学兽医学部兽医临床中心

犬由来第三世代セファロスポリン耐性大腸菌の全ゲノム解析と人との宿主間伝播の可能性の評価

犬源性第三代头孢菌素耐药大肠杆菌的全基因组分析及人际间宿主传播可能性评估

• 发表时间: 2022
• 作者: 西口真由美;堀田ほづみ;岡田みずほ,木下琢也;田浦直太;松本武浩;安木真世
• 通讯作者: 安木真世