Coordination project

协调项目

• 批准号: 314086261
• 负责人: Professor Dr. Utz Fischer
• 金额: --
• 依托单位: Lehrstuhl für Biochemie I
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/314086261?language=en
• 关键词: Coordination; project

Research of the past years has shown that post-transcriptional mechanisms have profound effects on gene expression. These mechanisms always regulate protein synthesis, either directly by affecting the rate of translation, or indirectly, by affecting the half-life or localization of mRNA. At all stages of their life, mRNAs interact with trans-acting factors, including proteins and various non-coding RNA, to serve their functions in gene expression. Because there are many mRNA-interacting factors and each mRNA is the blueprint of a particular protein, the resulting ribonucleoprotein particles (mRNPs) are unique in their composition. This gives rise to the mRNP code concept, which implies that specific sets of proteins, non-coding RNAs and other molecules bind to individual mRNAs and control their fate and function in every cell. The compositions of mRNPs as a bulk entity has been studied in some detail and several RNA binding proteins (RBPs) and their interaction sites on mRNA have been identified. However, insight into how the mRNP code manifests itself on individual transcripts and is read by the cell has remained limited. This initiative aims to bring together researchers from disciplines as diverse as systems biology, biochemistry, structural biology and bioinformatics to develop and apply methods allowing insight into the composition of mRNPs, how they are assembled and remodeled and how they function in specific cellular settings. To decipher the mRNP code, our overarching goal, we propose to combine data of individual disciplines and projects and subject them to a systematic computational analysis. This will enable the recognition of general principles and patterns of gene regulation by components of the mRNP. In the coordination project of this SPP, we apply for structural measures such as meetings, technical workshops and central facilities enabling the efficient cooperation of researchers of individual groups.

过去几年的研究表明，转录后机制对基因表达具有深远的影响。这些机制总是通过影响翻译速率直接调节蛋白质合成，或者通过影响 mRNA 的半衰期或定位间接调节蛋白质合成。在生命的各个阶段，mRNA 都会与反式作用因子（包括蛋白质和各种非编码 RNA）相互作用，以发挥其在基因表达中的功能。由于存在许多 mRNA 相互作用因子，并且每个 mRNA 都是特定蛋白质的蓝图，因此产生的核糖核蛋白颗粒 (mRNP) 的组成是独特的。这就产生了 mRNP 代码概念，它意味着特定的蛋白质组、非编码 RNA 和其他分子与单个 mRNA 结合并控制它们在每个细胞中的命运和功能。人们对 mRNA 作为整体实体的组成进行了一些详细的研究，并鉴定了几种 RNA 结合蛋白 (RBP) 及其在 mRNA 上的相互作用位点。然而，对 mRNP 代码如何在单个转录本上表现以及如何被细胞读取的了解仍然有限。该计划旨在汇集来自系统生物学、生物化学、结构生物学和生物信息学等不同学科的研究人员，开发和应用能够深入了解 mRNP 的组成、它们如何组装和重塑以及它们在特定细胞环境中如何发挥作用的方法。为了破译 mRNP 代码（我们的总体目标），我们建议结合各个学科和项目的数据，并对它们进行系统的计算分析。这将使我们能够认识 mRNP 成分的基因调控的一般原理和模式。在该SPP的协调项目中，我们申请了会议、技术研讨会和中央设施等结构性措施，使各个小组的研究人员能够高效合作。