Regulation of hemangioblst development by partial Epithelial Mesenchymal Transition

在2022财年，我们对SNAI2和ZEB2的过表达在谱系贡献(血管、血管、平滑肌谱系)中的作用进行了实验。我们发现这些EMT标记并不明显影响谱系标记基因的表达。然而，它们影响细胞谱系分离，很可能是在谱系指定之后。我们还研究了血管母细胞标志物(NPAS4L和ETV2，血管/血管标志物SC1/LMO2和平滑肌标志物HAND1/2的上游两个标志物)与EMT调节因子的关系。由于EMT发生在中胚层形成之前(在中胚层形成期间)和在造血细胞规范之后，并且至少SNAI2参与了原始条纹EMT和侧板中胚层EMT(本项目的主题)，我们正在梳理SNAI2、NPAS4L和SCL/LMO2/HAND2之间的上位关系。

In FY2022, we have performed experiments on effect of overexpression of Snai2 and Zeb2 in lineage contribution (blood, vessel, smooth muscle lineages). We have found that these EMT markers do not clearly affect lineage marker gene expression. However, they affect cell lineage segregation, likely after lineage specification. We have also investigated relationship between hemangioblast specifiers (NPAS4L and ETV2, two markers upstream of blood/vessel markers SCL/LMO2 and smooth muscle markers HAND1/2) and EMT regulators. As EMT occurs both before (during mesoderm formation) and after hemagioblast specification and at least SNAI2 is involved in both primitive streak EMT and lateral plate mesoderm EMT (subject of this project), we are in the process of sorting out epistatic relationship between SNAI2, NPAS4L, and SCL/LMO2/HAND2).