Regulation of hemangioblst development by partial Epithelial Mesenchymal Transition

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In FY2022, we have performed experiments on effect of overexpression of Snai2 and Zeb2 in lineage contribution (blood, vessel, smooth muscle lineages). We have found that these EMT markers do not clearly affect lineage marker gene expression. However, they affect cell lineage segregation, likely after lineage specification. We have also investigated relationship between hemangioblast specifiers (NPAS4L and ETV2, two markers upstream of blood/vessel markers SCL/LMO2 and smooth muscle markers HAND1/2) and EMT regulators. As EMT occurs both before (during mesoderm formation) and after hemagioblast specification and at least SNAI2 is involved in both primitive streak EMT and lateral plate mesoderm EMT (subject of this project), we are in the process of sorting out epistatic relationship between SNAI2, NPAS4L, and SCL/LMO2/HAND2).