Studies on the synthesis and biosynthesis of PR-Toxin

PR毒素的合成及生物合成研究

• 批准号: 317757081
• 负责人: Professor Dr. Jeroen Dickschat
• 金额: --
• 依托单位: Kekulé-Institut für Organische Chemie und Biochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/317757081?language=en
• 关键词: Studies; synthesis; biosynthesis; PR; Toxin

The unknown biosynthesis of the terpenoid PR-Toxin from the fungus Penicillium roqueforti will be elucidated. For this purpose a total synthesis of PR-Toxin via a route that also makes diverse hypothetical intermediates accessible will be developed. The prepared compounds will be used as analytical standards to identify potential intermediates in culture extracts by GC/MS and HPLC/MS. The potential intermediates will subsequently also be prepared carrying a 13C-labelling, and used in feeding experiments to distinguish intermediates from non-convertable biosynthetic shunt products. The question whether an isomerisation along the PR-Toxin biosynthesis is spontaneous or enzyme-catalysed will also be addressed in a suitable isotopic labelling experiment. The genes of the biosynthetic gene cluster for PR-Toxin will be cloned and heterologously expressed. The expressed proteins will be purified and used in incubation experiments with synthetic potential substrates. The insights obtained by the different strategies will be used to establish a robust model of the biosynthesis of PR-Toxin.

将阐明真菌Penicillium roqueforti的萜类PR毒素的未知生物合成。为此目的，将开发通过一种途径进行PR-毒素的全合成，该途径还可以获得各种假设的中间体。制备的化合物将用作分析标准品，以通过GC/MS和HPLC/MS鉴定培养物提取物中的潜在中间体。随后还将制备携带13 C标记的潜在中间体，并用于饲养实验，以区分中间体与不可转化的生物合成分流产物。在合适的同位素标记实验中也将解决沿着PR-毒素生物合成的异构化是自发的还是酶催化的问题。PR毒素生物合成基因簇的基因将被克隆并异源表达。表达的蛋白质将被纯化并用于与合成的潜在底物的孵育实验。通过不同策略获得的见解将用于建立PR-毒素生物合成的稳健模型。

项目成果

Volatiles from the ascomycete Daldinia cf. childiae (Hypoxylaceae), originating from China.

• DOI: 10.1039/c9md00083f
• 发表时间: 2019-05
• 期刊: MedChemComm
• 作者: Lukas Lauterbach;Tao Wang;M. Stadler;Jeroen S. Dickschat

Terpene Synthase Genes Originated from Bacteria through Horizontal Gene Transfer Contribute to Terpenoid Diversity in Fungi

• DOI: 10.1038/s41598-019-45532-1
• 发表时间: 2019-06-25
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Jia, Qidong;Chen, Xinlu;Chen, Feng
• 通讯作者: Chen, Feng

Volatiles from the hypoxylaceous fungi Hypoxylon griseobrunneum and Hypoxylon macrocarpum

来自缺氧真菌 Hypoxylon griseobrunneum 和 Hypoxylon macrocarpum 的挥发物

• DOI: 10.3762/bjoc.14.277
• 发表时间: 2018
• 期刊: Beilstein Journal of Organic Chemistry
• 影响因子: 2.7
• 作者: J. Rinkel;A. Babczyk;T. Wang;M. Stadler;J. S. Dickschat
• 通讯作者: J. S. Dickschat

Harzianone Biosynthesis by the Biocontrol Fungus Trichoderma

• DOI: 10.1002/cbic.201700462
• 发表时间: 2017-12-05
• 期刊: CHEMBIOCHEM
• 影响因子: 3.2
• 作者: Barra, Lena;Dickschat, Jeroen S.
• 通讯作者: Dickschat, Jeroen S.