分子認識PEGゲルキャピラリー電気泳動による生体分子の構造・サイズ選択的分離

通过分子识别 PEG 凝胶毛细管电泳对生物分子进行结构和尺寸选择性分离

• 批准号: 20J15151
• 负责人: 劉 晨晨
• 金额: $ 1.22万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-24 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20J15151/
• 关键词: poly(ethylene glycol); protein analysis; affinity electrophoresis; electrophoresis; DNA analysis

The poly(ethylene glycol) (PEG) based molecularly imprinted hydrogel (MIH) was fabricated for the molecular recognition of the target protein. Copoly(PEG acrylate/PEG diacrylate) (copoly(PEGA/PEGDA)) hydrogel was employed in the preparation of MIH for cytochrome c recognition. A highly specific molecular recognition was realized when using the MIH as a sorbent, evidenced by (1) the MIH showed adsorption to the cytochrome c target while the non-imprinted hydrogel showed no adsorption to the target, and (2) the MIH hydrogel showed higher adsorption to the cytochrome c target than to the other unspecific proteins. When tested as the affinity substance in electrophoresis, the MIH retarded the migration of cytochrome c to a higher extent compared with the other unspecific proteins.

制备了基于聚乙二醇（PEG）的分子印迹水凝胶（MIH），用于靶蛋白的分子识别。采用聚（聚乙二醇丙烯酸酯/聚乙二醇二丙烯酸酯）（聚（聚乙二醇/聚乙二醇））水凝胶制备用于细胞色素c识别的MIH。使用MIH作为吸附剂时，实现了高度特异性的分子识别，证明：(1)MIH对细胞色素c目标具有吸附作用，而非印迹水凝胶对目标没有吸附作用；(2)MIH水凝胶对细胞色素c目标的吸附作用高于其他非特异性蛋白质。在电泳中作为亲和物质检测时，与其他非特异性蛋白相比，MIH在更大程度上延缓了细胞色素c的迁移。

项目成果

Study of Copoly(poly(ethylene glycol) diacrylate/poly(ethylene glycol) acrylate) Hydrogel as a Molecularly Imprinted Polymer for Cytochrome c Recognition

共聚(聚(乙二醇)二丙烯酸酯/聚(乙二醇)丙烯酸酯)水凝胶作为分子印迹聚合物用于细胞色素c识别的研究

• 发表时间: 2021
• 作者: Chenchen Liu;Takuya Kubo;Koji Otsuka
• 通讯作者: Koji Otsuka

Controllable molecular sieving effect in capillary electrophoresis by tuning the network structure of poly (ethylene glycol)-based hydrogels

通过调节聚乙二醇水凝胶的网络结构来控制毛细管电泳中的分子筛分效果

• 发表时间: 2021
• 作者: Chenchen Liu;Toyohiro Naito;Takuya Kubo;Koji Otsuka
• 通讯作者: Koji Otsuka

Controllable Molecular Sieving by copoly(Poly(ethylene glycol) Acrylate/Poly(ethylene glycol) Diacrylate)-Based Hydrogels via Capillary Electrophoresis for DNA Fragments

通过基于共聚（聚（乙二醇）丙烯酸酯/聚（乙二醇）二丙烯酸酯）的水凝胶通过毛细管电泳进行 DNA 片段的可控分子筛分

• DOI: 10.1021/acsapm.0c00567
• 发表时间: 2020-09-11
• 期刊: ACS APPLIED POLYMER MATERIALS
• 影响因子: 5
• 作者: Liu, Chenchen;Kubo, Takuya;Otsuka, Koji
• 通讯作者: Otsuka, Koji

Hydrogels in Electrophoresis: Applications and Advances

• DOI: 10.2116/analsci.20r004
• 发表时间: 2020-10
• 期刊: Analytical Sciences
• 影响因子: 1.6
• 作者: Chenchen Liu;T. Kubo;K. Otsuka

Fabrication of Hydrogels for the Recognition of Specified Proteins via Grafting Molecularly Imprinted Polymers

通过接枝分子印迹聚合物制备水凝胶来识别特定蛋白质

• 发表时间: 2021
• 作者: Chenchen Liu;Takuya Kubo;Koji Otsuka
• 通讯作者: Koji Otsuka