Novel Small Cantilever

新型小悬臂

• 批准号: 327297123
• 负责人: Professor Dr.-Ing. Thomas Fröhlich
• 金额: --
• 依托单位: Institut für Prozessmess- und Sensortechnik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/327297123?language=en
• 关键词: Novel; Small; Cantilever

Atomic Force Microscopy (AFM) allows for micrographs of a millionfold magnification and hence visualization of single molecules and even atoms. During the last 25 years it has become a standard analytics tool in a number of research fields. For future applications in biotechnology or life sciences, there are two requirements of particular importance: For the display of biological processes in and at living cells, a frame rate comparable with the speed of the proceeding processes is required. Therefore cantilevers with a high resonance frequency are needed. At the same time, these cantilevers have to have a low spring constant, to avoid a mechanical damaging of cells or biofilms.Furthermore, such cantilevers are used to measure mechanical properties such as the stiffness of biological cells (e.g. force spectroscopy of cancer cells). For truly quantitative and comparable force measurements, a traceable calibration of the spring constants with low uncertainties is urgently required. At present, no cantilevers with both high resonance frequencies and low spring constants are available, which are in addition calibrated traceable with low forces. The required high resolving calibration systems are not available at present.The proposed project addresses exactly these missing items. Both novel small cantilevers with both high resonance frequencies and low spring constants, as well as a new high resolving measurement method for the investigation of the links and the calibration of the spring constants shall be developed and researched.The fundamental discrepancy between a high resonance frequency and a low spring constant at the same time, shall be dissolved by the development of novel small cantilevers with small dimensions and hence a low mass.The required high force resolution of the measurement and calibration system for the spring constants shall be achieved by a simplification of its mechanics and consequently an increase of the force sensitivity and decrease of sensitivity to disturbances.

原子力显微镜(AFM)可以使显微照片放大一百万倍，从而使单个分子甚至原子可视化。在过去的25年里，它已经成为许多研究领域的标准分析工具。对于未来在生物技术或生命科学中的应用，有两个特别重要的要求：对于在活细胞内和在活细胞上显示生物过程，需要与进行过程的速度相媲美的帧速率。因此，需要具有较高共振频率的悬臂梁。同时，这些悬臂必须具有较低的弹性常数，以避免对细胞或生物膜的机械破坏。此外，这些悬臂用于测量生物细胞的力学性能(例如，癌细胞的力谱)。对于真正定量和可比较的力测量，迫切需要对低不确定度的弹簧常数进行可跟踪校准。目前，没有同时具有高共振频率和低弹簧常数的悬臂可供选择，此外，还可以用低作用力校准可追踪的悬臂。目前还没有所需的高分辨率校准系统。拟议的项目恰恰解决了这些缺失的问题。将开发和研究具有高共振频率和低弹簧常数的新型小悬臂，以及用于连杆研究和弹簧常数校准的新的高分辨率测量方法。高共振频率和低弹簧常数之间的根本差异将通过开发小尺寸和低质量的新型小悬臂来解决。对弹簧常数测量和校准系统所需的高精度力分辨率应通过简化其机械结构来实现，从而提高力灵敏度和对干扰的灵敏度。

项目成果

Towards alternative 3D nanofabrication in macroscopic working volumes

• DOI: 10.1088/1361-6501/aadb57
• 发表时间: 2018-10
• 期刊: Measurement Science and Technology
• 影响因子: 2.4
• 作者: M. Kühnel;T. Frohlich;R. Füßl;M. Hoffmann;E. Manske;I. Rangelow;J. Reger;C. Schäffel;S. Sinzinger;Jens-Peter Zöllner