In vivo imaging and optical manipulation of the small intestinal mucosa using 2-photon microscopy and femtosecond laser nano surgery

使用双光子显微镜和飞秒激光纳米手术对小肠粘膜进行体内成像和光学操作

• 批准号: 36474205
• 负责人: Professor Dr. Andreas Gebert
• 金额: --
• 依托单位: Institut für Biomedizinische Optik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/36474205?language=en
• 关键词: vivo; imaging; optical; manipulation; small

Novel methods for intravital 2-photon microscopy based on tissue auto-fluorescence have successfully been established in the first funding period. In a newly developed mouse model, we discovered several previously unknown processes of epithelial cell biology and mucosal immunology. For the first time, the dynamics of intraepithelial lymphocytes was observed and their kinetics quantified. Integration of a UV manipulation laser and development of an online dosimetry made it possible to cause highly localized tissue damage and examine the in vivo tissue responses. In the present renewal, the non-linear imaging range will be shifted to the infrared, endogenous and exogenous fluorophores will be excited quasi-simultaneously at multiple wavelengths, the focus quality will be improved by adaptive optics, and laser nano-surgery controlled by integrated bubble interferometry will be established. Using these new imaging techniques, mechanisms of lymphocyte migration and antigen processing in the gut epithelium will be studied. Controlled laser manipulation will provide new insight into mechanisms involved in tissue damage and repair. Alterations of tissue dynamics in inflammatory disease will be investigated by establishing an inflammation model for the small intestinal mucosa, directly observing the invasion of bacteria, and tracking immune cells and tissue repair after local damage.

基于组织自发荧光的活体双光子显微镜的新方法已在第一个资助期内成功建立。在一个新开发的小鼠模型中，我们发现了一些以前未知的上皮细胞生物学和粘膜免疫学过程。第一次，上皮内淋巴细胞的动力学进行了观察和量化。紫外操纵激光器的集成和在线剂量测定的发展使得有可能引起高度局部化的组织损伤并检查体内组织反应。在目前的更新中，非线性成像范围将转移到红外线，内源性和外源性荧光团将在多个波长下准同时激发，自适应光学将提高聚焦质量，并将建立由集成气泡干涉法控制的激光纳米手术。使用这些新的成像技术，淋巴细胞迁移和抗原处理在肠道上皮细胞的机制将进行研究。受控激光操作将为组织损伤和修复机制提供新的见解。通过建立小肠粘膜炎症模型，直接观察细菌的侵入，并跟踪局部损伤后的免疫细胞和组织修复，研究炎症性疾病中组织动力学的变化。

项目成果

Wavelength dependence of femtosecond laser-induced breakdown in water and implications for laser surgery

• DOI: 10.1103/physrevb.94.024113
• 发表时间: 2016-07-18
• 期刊: PHYSICAL REVIEW B
• 影响因子: 3.7
• 作者: Linz, Norbert;Freidank, Sebastian;Vogel, Alfred
• 通讯作者: Vogel, Alfred

Intravital autofluorescence 2-photon microscopy of murine intestinal mucosa with ultra-broadband femtosecond laser pulse excitation: image quality, photodamage, and inflammation

采用超宽带飞秒激光脉冲激发的小鼠肠粘膜活体自发荧光双光子显微镜：图像质量、光损伤和炎症

• DOI: 10.1117/1.jbo.20.11.116001
• 发表时间: 2015
• 期刊: Journal of Biomedical Optics
• 影响因子: 3.5
• 作者: Klinger A;Krapf L;Orzekowsky-Schroeder R;Koop N;Vogel A;Hüttmann G
• 通讯作者: Hüttmann G

A new nanosecond UV laser at 355 nm: early results of corneal flap cutting in a rabbit model.

新型 355 nm 纳秒紫外激光：兔模型角膜瓣切割的早期结果

• DOI: 10.1167/iovs.13-12580
• 发表时间: 2013
• 期刊: Investigative ophthalmology & visual science
• 影响因子: 4.4
• 作者: Trost A;Schrödl F;Strohmaier C;Bogner B;Runge C;Kaser-Eichberger A;Vogel A;Linz N;Freidank S;Hilpert A;Zimmermann I;Grabner G;Reitsamer HA
• 通讯作者: Reitsamer HA

Alternativen zur Femtosekundentechnologie

飞秒技术的替代方案

• DOI: 10.1007/s00347-013-2994-8
• 发表时间: 2014
• 期刊: Der Ophthalmologe
• 作者: Vogel A;Freidank S;Linz N
• 通讯作者: Linz N