200kV Cryo Transmission Electron Microscope

200kV冷冻透射电镜

• 批准号: 386367113
• 金额: --
• 依托单位: Ruprecht-Karls-Universität Heidelberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/386367113?language=en
• 关键词: 200kV; Cryo; Transmission; Electron; Microscope

With the current proposals two lines of electron microscopy studies will be strongly enhanced at Heidelberg Campus: With two more cryo TEMs (one low-end and one medium/high-end) a stringent sample workflow is established, which allows the screening of a large number of samples for Single Particle Analysis (SPA) before using time for ultimate-resolution imaging at our existing high-end cryo TEM. Such an expansion of microscope time for SPA is absolutely necessary for a planned professorship in macromolecular biochemistry – which will further add to the increasing demands of all biomedical institutes. At the same time, such a screening workflow on supporting microscopes will reduce the workload on our existing high-end cryo instrument and will free time for additional Cryo Electron Tomography (CET) studies. With the hiring of additional junior faculty it is mandatory to increase instrument time for such work. We recently upgraded our dedicated high-end cryo TEM with a post energy filter Direct Electron Detector, which now will allow state-of-the-art cryo CET work. However, it is also most important to establish the necessary cryo-lamella sample preparation platform to image macromolecular complexes in their cellular environment, as well as to optimize tomographic data collection by adding most advanced physical phase plate technology. In the end, this latter optimization will also help any macromolecular imaging work, in particular the planned studies on small protein complexes.

根据目前的提议，海德堡校区的两条电子显微镜研究将得到大力加强：通过另外两台冷冻 TEM（一台低端和一台中/高端），建立了严格的样品工作流程，允许在我们现有的高端冷冻 TEM 上进行最终分辨率成像之前筛选大量样品进行单颗粒分析 (SPA)。如此延长 SPA 的显微镜时间对于计划设立的大分子生物化学教授职位来说是绝对必要的，这将进一步增加所有生物医学机构日益增长的需求。与此同时，这种支持显微镜的筛选工作流程将减少我们现有高端冷冻仪器的工作量，并为额外的冷冻电子断层扫描 (CET) 研究腾出时间。随着雇用更多的初级教师，必须增加用于此类工作的仪器时间。我们最近升级了我们的专用高端冷冻 TEM，配备了后能量滤波器直接电子探测器，现在可以进行最先进的冷冻 CET 工作。然而，最重要的是建立必要的冷冻片层样品制备平台，以对细胞环境中的大分子复合物进行成像，并通过添加最先进的物理相板技术来优化断层扫描数据收集。最后，后一种优化也将有助于任何大分子成像工作，特别是计划中的小蛋白质复合物研究。