Cryo-Transmission Electron Microscope 200 kV

冷冻透射电子显微镜 200 kV

• 批准号: 450084146
• 金额: --
• 依托单位: Goethe-Universität Frankfurt am Main
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/450084146?language=en
• 关键词: Cryo; Transmission; Electron; Microscope; 200

Research aims at understanding dynamic protein assemblies and machineries that use the energy of ATP to control complex cellular processes. Integrated structural biology plays an important role in our research approaches by visualizing molecular machines in different conformations. A screening cryo transmission electron microscope (TEM) 200kV is requested for current research projects and future activities. Due to the limited access to high-end microscope of this type, there is an urgent need for a cryo-TEM at the Goethe University Frankfurt. The instrument complements the ten-year-old Krios I with a 62.5% Uni share, which is mainly used for electron tomography. The Cryo-TEM is a device that meets the highest requirements in terms of resolution, efficient sample analysis, and universal user-friendliness. A corresponding cryo-EM infrastructure is available at the location, which ensures immediate and quick commissioning and also guarantees the working groups involved in the application process an efficient use and a wide range of users.

研究旨在了解动态蛋白质组装和使用ATP能量控制复杂细胞过程的机制。整合结构生物学通过可视化不同构象的分子机器在我们的研究方法中发挥着重要作用。目前的研究项目和未来的活动需要一个筛选低温透射电子显微镜（TEM）200千伏。由于这种类型的高端显微镜有限，法兰克福歌德大学迫切需要低温TEM。该仪器补充了十年的Krios I，拥有62.5%的Uni份额，主要用于电子断层扫描。Cryo-TEM是一种在分辨率、高效样品分析和通用用户友好性方面满足最高要求的设备。相应的低温EM基础设施在现场可用，这确保了即时和快速的调试，并保证了应用过程中所涉及的工作组的有效使用和广泛的用户。