Cryo Transmission Electron Microscope for SPA, Cryo-ET and MicroED studies at UCSB
UCSB 用于 SPA、Cryo-ET 和 MicroED 研究的冷冻透射电子显微镜
基本信息
- 批准号:10177740
- 负责人:
- 金额:$ 200万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-07-01 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:AppointmentArchitectureAreaAutomobile DrivingBiochemistryBiocompatible MaterialsBiologicalBiologyBiomedical EngineeringCaliforniaCharacteristicsChemistryCommunitiesCryoelectron MicroscopyElectron MicroscopeElectron Microscopy FacilityEngineeringFacultyFundingKnowledgeLaboratoriesLeadershipLifeMicro Electron DiffractionMolecularMolecular ChaperonesNucleic AcidsOperations ResearchPermeabilityProcessProteinsResearchResolutionRunningScienceStructural BiochemistryStructureSystemTalentsTherapeuticUnited States National Institutes of HealthUniversitiesVisualizationbasebiological systemscollegedoctoral studentelectron tomographyinhibitor/antagonistinsightinstrumentationinterdisciplinary collaborationparticleprogramsprotein functionstructural biologytransmission process
项目摘要
PROJECT SUMMARY/ABSTRACT
With the purchase of a GlaciosTM transmission electron microscope system we seek to establish the first single particle
analysis (SPA) cryo-electron microscopy (cryo-EM), -electron tomography (-ET) and micro-electron diffraction (micro-
ED) facility at the University of California Santa Barbara, and the only one on the California Central Coast: the UCSB
Structural Biology cryo-Electron Microscopy (SB2EM) facility. Dedicated biological cryo-EM capabilities will become the
linchpin to UCSB’s strong leadership and strength in quantitative biology, structural biochemistry and bioengineering at the
interdisciplinary interface. Nowhere is the boundary between Departments, Colleges and Programs more permeable than at
UCSB: faculty advise PhD students of all Science and Engineering Departments with no to little barriers, dual appointments
in more than one department is the norm at UCSB and research operations relying on high-end and state-of-the-art
instrumentation in professionally run shared facilities is a forte of the UCSB campus. The SB2EM facility will be integrated
into this culture of interdisciplinary collaboration, as well as a network of first-rate shared facilities and laboratories across
our campus’s largely merged science and engineering community. The impact of SB2EM on the current NIH funded UCSB
faculty will be direct, immediate and dramatic, but it will also go well beyond the currently funded NIH projects by inspiring
and enabling entirely new areas of biomedically inspired research, and the hiring of new faculty talent.
The ability to the “turn the lights on” to directly see the molecular machinery of life fundamentally changes how we
understand and, ultimately, manipulate biological materials, systems and processes. Cryo-EM brings atomic- and molecular-
level information, typically the core approach of a chemist, into biology and bioengineering, and vice versa, through, for
example, the visualization of how molecular chaperones untangle neurofibrillary plaques, or through the structure-based
engineering of molecular inhibitors and architectures built of protein and nucleic acid building blocks. The emergence of
unprecedentedly high-resolution structural insights into biological molecules and systems offers a critical competitive
advantage towards understanding their functions, discovering function-driving mechanisms, and engineering therapeutic
solutions. The study of the protein function will be dramatically facilitated and the re-engineering of biological systems
vastly enhanced with knowledge of the underlying structure and structural characteristics. It is this insight that led us to
prioritize structure-based biochemistry, bioorganic chemistry, biology, and bioengineering at UCSB.
项目总结/摘要
通过购买GlaciosTM透射电子显微镜系统,我们试图建立第一个单粒子
分析(SPA)、低温电子显微镜(cryo-EM)、电子断层扫描(-ET)和微电子衍射(micro-electron diffraction)。
艾德)设施,以及加州中央海岸唯一的一个:UCSB
结构生物学冷冻电子显微镜(SB 2 EM)设施。专用的生物冷冻EM能力将成为
关键UCSB的强大的领导和力量在定量生物学,结构生物化学和生物工程在
跨学科界面没有什么地方是部门之间的界限,学院和程序比在渗透
UCSB:教师建议所有科学和工程系的博士生没有到很少的障碍,双重任命
在一个以上的部门是UCSB和研究业务的规范依赖于高端和最先进的
在专业运行的共享设施的仪器是UCSB校园的强项。SB 2 EM设施将被整合
这种跨学科合作的文化,以及一流的共享设施和实验室网络,
我们校园的科学和工程社区基本上合并了。SB 2 EM对当前NIH资助的UCSB的影响
教师将是直接的,即时的和戏剧性的,但它也将远远超出目前资助的NIH项目,通过激励
并实现生物医学启发研究的全新领域,以及雇用新的教师人才。
“打开灯”直接看到生命分子机制的能力从根本上改变了我们
理解并最终操纵生物材料、系统和过程。Cryo-EM将原子和分子
水平的信息,通常是化学家的核心方法,进入生物学和生物工程,反之亦然,通过,
例如,分子伴侣如何解开神经元斑块的可视化,或者通过基于结构的
分子抑制剂的工程化以及由蛋白质和核酸构建块构建的结构。的出现
对生物分子和系统的前所未有的高分辨率结构洞察提供了一个关键的竞争优势。
有利于了解它们的功能,发现功能驱动机制,并工程治疗
解决方案蛋白质功能的研究将大大促进生物系统的重新设计,
随着对底层结构和结构特征的了解而大大增强。正是这种洞察力让我们
优先考虑UCSB的基于结构的生物化学,生物有机化学,生物学和生物工程。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
DORIT HANEIN其他文献
DORIT HANEIN的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('DORIT HANEIN', 18)}}的其他基金
Structure and function of the Plasmodium myosin XIV-actin glideosome
疟原虫肌球蛋白 XIV 肌动蛋白滑胶体的结构和功能
- 批准号:
9363011 - 财政年份:2017
- 资助金额:
$ 200万 - 项目类别:
Structure and function of the Plasmodium myosin XIV-actin glideosome
疟原虫肌球蛋白 XIV 肌动蛋白滑胶体的结构和功能
- 批准号:
9913454 - 财政年份:2017
- 资助金额:
$ 200万 - 项目类别:
Molecular mechanism of BCL2-dependent apoptosis
BCL2依赖性细胞凋亡的分子机制
- 批准号:
8856525 - 财政年份:2014
- 资助金额:
$ 200万 - 项目类别:
International Conference on Image Analysis in Three-dimensional Cryo-EM
三维冷冻电镜图像分析国际会议
- 批准号:
8785968 - 财政年份:2014
- 资助金额:
$ 200万 - 项目类别:
Molecular mechanism of BCL2-dependent apoptosis
BCL2依赖性细胞凋亡的分子机制
- 批准号:
8702959 - 财政年份:2014
- 资助金额:
$ 200万 - 项目类别:
Instrumentation Upgrade: acquisition of an intermediate voltage TEM
仪器升级:获取中间电压 TEM
- 批准号:
8335214 - 财政年份:2012
- 资助金额:
$ 200万 - 项目类别:
Ultrastructural Basis of Mechanotransduction in Matrix Adhesions
基质粘附力传导的超微结构基础
- 批准号:
8550088 - 财政年份:2011
- 资助金额:
$ 200万 - 项目类别:
Ultrastructural Basis of Mechanotransduction in Matrix Adhesions
基质粘附力传导的超微结构基础
- 批准号:
8165563 - 财政年份:2011
- 资助金额:
$ 200万 - 项目类别:
Ultrastructural Basis of Mechanotransduction in Matrix Adhesions
基质粘附力传导的超微结构基础
- 批准号:
8333958 - 财政年份:2011
- 资助金额:
$ 200万 - 项目类别:
相似海外基金
Practical Study on Disaster Countermeasure Architecture Model by Sustainable Design in Asian Flood Area
亚洲洪泛区可持续设计防灾建筑模型实践研究
- 批准号:
17K00727 - 财政年份:2017
- 资助金额:
$ 200万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional architecture of a face processing area in the common marmoset
普通狨猴面部处理区域的功能架构
- 批准号:
9764503 - 财政年份:2016
- 资助金额:
$ 200万 - 项目类别:
Heating and airconditioning by hypocausts in residential and representative architecture in Rome and Latium studies of a phenomenon of luxury in a favoured climatic area of the Roman Empire on the basis of selected examples.
罗马和拉齐奥的住宅和代表性建筑中的火烧供暖和空调根据选定的例子,研究了罗马帝国有利的气候地区的奢华现象。
- 批准号:
317469425 - 财政年份:2016
- 资助金额:
$ 200万 - 项目类别:
Research Grants
SBIR Phase II: Area and Energy Efficient Error Floor Free Low-Density Parity-Check Codes Decoder Architecture for Flash Based Storage
SBIR 第二阶段:用于基于闪存的存储的面积和能源效率高、无错误层的低密度奇偶校验码解码器架构
- 批准号:
1632562 - 财政年份:2016
- 资助金额:
$ 200万 - 项目类别:
Standard Grant
SBIR Phase I: Area and Energy Efficient Error Floor Free Low-Density Parity-Check Codes Decoder Architecture for Flash Based Storage
SBIR 第一阶段:用于基于闪存的存储的面积和能源效率高、无错误层低密度奇偶校验码解码器架构
- 批准号:
1520137 - 财政年份:2015
- 资助金额:
$ 200万 - 项目类别:
Standard Grant
A Study on The Spatial Setting and The Inhavitant's of The Flood Prevention Architecture in The Flood Area
洪泛区防洪建筑空间设置及居民生活研究
- 批准号:
26420620 - 财政年份:2014
- 资助金额:
$ 200万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Area and power efficient interconnect architecture for multi-bit processing on FPGAs
用于 FPGA 上多位处理的面积和功率高效互连架构
- 批准号:
327691-2007 - 财政年份:2011
- 资助金额:
$ 200万 - 项目类别:
Discovery Grants Program - Individual
A FUNDAMENTAL STUDY ON UTILIZATION OF THE POST-WAR ARCHITECTURE AS URBAN REGENERATION METHOD, A case of the central area of Osaka city
战后建筑作为城市更新方法的基础研究——以大阪市中心区为例
- 批准号:
22760469 - 财政年份:2010
- 资助金额:
$ 200万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Area and power efficient interconnect architecture for multi-bit processing on FPGAs
用于 FPGA 上多位处理的面积和功率高效互连架构
- 批准号:
327691-2007 - 财政年份:2010
- 资助金额:
$ 200万 - 项目类别:
Discovery Grants Program - Individual
Area and power efficient interconnect architecture for multi-bit processing on FPGAs
用于 FPGA 上多位处理的面积和功率高效互连架构
- 批准号:
327691-2007 - 财政年份:2009
- 资助金额:
$ 200万 - 项目类别:
Discovery Grants Program - Individual