Development of Method for Evaluating Safety of Water Applying PCR

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PCR method was developed as a quantitative detection of viruses in water, including the investigation of pre-treatment method. Application of PCR method to the water treated by a treatment process (Ultraviolet Irradiation). RNA phage Q-beta was used for the experiment as a model virus.In order to inprove the quantitatative PCR,optimisation of beffer concentration in both reverse transcription and DNA amplify, and development of nested PCR were done. Result showed the detection limit was 1 PFU.It was suggested that using computer program to calculate MPN lead to the utilization of quantitative PCR date.Effect of ultraviolet disinfection on the PCR detection was also investigated. Value of surviving phage concentration measured by conventional plapue method and that measured by PCR method were compared. Result showed that PCR measurment detected most of inactivated phages. Quantity of detection was assumed to depend on the ratio of the length of target RNA of PCR detection to the whole lengh of phage RNA.These results showed that PCR method was hard to be applied to the monitoring the performance of ultraviolet disinfection. Further research was required to utilize the PCR to measure the safety of water.