Evaluation of Water Treatment Processes Using Bacteriophages as Tracer

使用噬菌体作为示踪剂评价水处理过程

• 批准号: 62850101
• 负责人: OHGAKI Shinichiro
• 金额: $ 4.16万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62850101/
• 关键词: Bacteriophage; Model Virus; Chlorination; Activated Sludge Particulate; Plaque Size; Composting Process; Inactivation of Virus; Coliphage QBETA; プラ-クサイズ; 大腸菌ファージ; 活性汚泥への吸着; 生物膜への吸着; ウィルスの不活化; コンポスト化

A new method was developed to evaluate the removal efficiency of viruses in water treatment processes using coliphages as a tracer. The results of this research are as follow. 1. Most of coliphages in sewage were found to be adsorbed onto suspended solids. The adsorbed coliphages could be easily eluted into the liquid phase with beef extract. The adsorption mechanism of viruses onto activated sludge particles was investigated in the laboratory-scale batch experiment using coliphage QBETA as model virus. The pH value and the microbial activity were found to be the influential factors. Coliphage QBETA was used also in the chlorination process and the ultraviolet irradiation process as model virus to evaluate the virus inactivation efficiency. 2. The biofilm process had less removal efficiency of coliphages than activated sludge process. Coliphages were adsorbed onto the biofilms and survived there without inactivation. Classification of coliphages was attempted on the plaque size (-1mm, 1-3mm, 3mm-) using E.coli B as a host organism in the activated sludge process. 3. The survival and inactivation of viruses in the composting process was investigated using coliphage QBETA as a model virus. The inactivation process of QBETA in the compost was expressed using first order kinetics considering temperature and water content.

以大肠杆菌为示踪剂，建立了一种评价水处理过程中病毒去除效果的新方法。研究结果如下。1.污水中的大肠杆菌大多吸附在悬浮物上。吸附后的大肠杆菌可以用牛肉膏洗脱到液相中。以大肠杆菌噬菌体QBETA为模型病毒，通过实验室规模的批式试验研究了活性污泥颗粒对病毒的吸附机理。pH值和微生物活性是影响发酵效果的主要因素。大肠杆菌噬菌体QBETA也被用于氯化工艺和紫外线照射工艺中作为模型病毒来评价病毒灭活效率。2.生物膜法对大肠杆菌的去除效果不如活性污泥法。大肠杆菌被吸附到生物膜上，并在那里存活而不失活。在活性污泥法中，使用大肠杆菌B作为宿主生物，尝试根据噬菌斑大小（-1 mm、1- 3 mm、3 mm-）对大肠杆菌进行分类。3.以大肠杆菌噬菌体QBETA为模型病毒，研究了堆肥过程中病毒的存活和灭活。QBETA在堆肥中的失活过程表示为考虑温度和含水量的一级动力学。

项目成果

Ketratanakul.A: Water Pollution Control in Asia,Pergamon. 321-327 (1988)

Ketratanakul.A：亚洲水污染控制，佩加蒙。

ケトラタナクル(ketratanakul,A): "Occurrence of Coliphages and RNA-F-Specific Coliphages in Sewages" 環境科学シンポジウム講演報告集. 52 (1987)

Ketratanakul (A)：“污水中大肠杆菌噬菌体和 RNA-F 特异性大肠杆菌噬菌体的出现”环境科学研讨会讲座报告 52 (1987)。

大村達夫: "下水処理場における指標微生物の除去" 衛生工学研究論文集. vol.25. 129-137 (1989)

Tatsuo Omura：“污水处理厂中指示微生物的去除”卫生工程研究杂志第 25 卷（1989 年）。

Ohgaki, S., Nagaoka, H., Sekiya, T, and Izumino, M.: "Evaluation of Ultraviolet Light Disinfection Design Using Bacteriophages as Bioindicator." Selected Topics on Clean Technology, Proceedings of Workshop on Clean Technology, Asian Institute of Technolog

Ohgaki, S.、Nagaoka, H.、Sekiya, T 和 Izumino, M.：“使用噬菌体作为生物指示剂的紫外线消毒设计的评估”。

Ketratanakul.A: "Adsorption of RNA-F-specific coliphages Qβ,on Suspended solids in activated sludge" Water Pollution Control in Asia,Pergamon. 321-327 (1988)

Ketratanakul.A：“RNA-F 特异性大肠杆菌噬菌体 Qβ 在活性污泥中悬浮固体上的吸附”，亚洲水污染控制，Pergamon 321-327 (1988)。