Quantitative microbiological technology for advanced management for water environment

水环境先进管理的定量微生物技术

• 批准号: 12305032
• 负责人: OHGAKI Shinichiro
• 金额: $ 21.02万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12305032/
• 关键词: Water microbiology; quantification; gene technology; PCR; FISH; antibiotics; VIRUS; concentration; 微生物; 水環境; 上下水道; 遺伝子; 酵素

There had been a great progress in genetic engineering and analytical methods for water chemistry in the last decade of 20th century. However, these techniques were not directly applied to the field of the environmental engineering, because it includes such difficulty in understanding the phenomena that low concentration and complicated microbial community while many other compounds are interfere the analytical methods. In this study, in order to utilize the emerging technology for environmental engineering for water management, various efforts were paid for quantification of microbes for the purpose of elucidation of complex microbial community and the fates of pathogenic microbes in water environment.

世纪的最后十年，基因工程和水化学分析方法取得了巨大的进展。然而，这些技术并没有直接应用于环境工程领域，因为它包括这样的困难，理解的现象，低浓度和复杂的微生物群落，而许多其他化合物干扰的分析方法。在这项研究中，为了利用新兴的环境工程技术的水管理，各种努力付出的微生物的定量的目的是阐明复杂的微生物群落和病原微生物在水环境中的命运。

项目成果

Alam, M.Z.B., Luxmy, B.S., Katayama, H., Ohgaki, S.: "Detecting the Viability of Particle Associated Bacteria after UV-radiation: A Molecular Biological Approach"Proc. of First Asian Pacific Regional Conference of IWA, Fukuoka. 661-666 (2001)

Alam, M.Z.B.、Luxmy, B.S.、Katayama, H.、Ohgaki, S.：“检测紫外线辐射后颗粒相关细菌的活力：分子生物学方法”Proc。

Imachi, H., Sekiguchi, Y., Kamagata, Y., Ohashi, A., Harada, H.: "Cultivation and In Situ Detection of a Thermophilic Bacterium Capable of Oxidizing Propionate in Syntrophic Association with Hydrogenotropnic Methanogens in a Thermophilic Methanogenie Gran

Imachi, H.、Sekiguchi, Y.、Kamagata, Y.、Ohashi, A.、Harada, H.：“在嗜热产甲烷菌中与嗜氢产甲烷菌共养结合的能够氧化丙酸的嗜热细菌的培养和原位检测

Sei K., Nakao M., Mori K., Ike M., Kohno T., Fujita M: "Design of PCR primers and a gene probe for extensive detection of poly(3-hydroxybutyrate)(PHB)-degrading bacteria possessing fibronectin type III linker type-PHB depolymerases"Applied Microbiology an

Sei K.、Nakao M.、Mori K.、Ike M.、Kohno T.、Fujita M：“PCR 引物和基因探针的设计，用于广泛检测具有纤连蛋白的聚（3-羟基丁酸酯）（PHB）降解细菌

Sekiguchi, Takahashi, H., Y., Kamagata, Y, Ohashi, A., Harada, H.: "In Situ Detection, Isolation, and Physiological Properties of a Thin Filamentous Microorganism Abundant in Methanogenic Granular Sluges: a Novel Isolate Affiliated with a Clone Cluster, t

Sekiguchi，Takahashi，H.，Y.，Kamagata，Y，Ohashi，A.，Harada，H.：“产甲烷颗粒污泥中丰富的细丝状微生物的原位检测、分离和生理特性：一种与

Katayama H., M. Nakamura and S. Ohgaki: "PCR determination of inactivated RNA colipnage Qβ, Advances in Water and Wastewater Treatment, Edited by Matsuo, T., Hanaki, K., Satoh, H."Elsevier. 101-108 (2001)

Katayama H.、M. Nakamura 和 S. Ohgaki：“灭活 RNA colipnage Qβ 的 PCR 测定，水和废水处理进展，由 Matsuo, T.、Hanaki, K.、Satoh, H. 编辑”Elsevier 101-108。 (2001)