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Bacteriophages as model viruses for water disinfection processes

噬菌体作为水消毒过程的模型病毒

基本信息

批准号：
04555135
负责人：
OHGAKI Shinichiro
金额：
$ 5.76万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The possibility of multiplication of F-specific RNA phage (FRNA phage) mulitiplication in the environment was investigated. Using the QB strain in pure culture as a model FRNA phage, the effects of bacterial growth phage, substrate concentration and cultivation temperature on phage multiplication were studied. Similar experiments with environmental samples from raw sewage were then preformed.Viricidal effect of ozone was investigated in an ozone demand-free batch system, using F-specific RNA coliphage QB as a model virus. Inactivation of QB did not follow the first order kinetics.Rejection of heterotrophic and coliphages were studied by using hollow fiber membrane. Indigenous RNA phages in intake water of a water purification plant were dectected in the filtrate while DNA phages which kave larger diametes than RNA phages were not detected.For adopting phages as model viruses for water quality control, RNA phages seems more reasonable.

研究了F特异性RNA噬菌体（FRNA噬菌体）在环境中增殖的可能性。以QB菌株纯培养物为FRNA噬菌体模型，研究了菌体生长、底物浓度和培养温度对噬菌体增殖的影响。以F-特异性RNA大肠杆菌噬菌体QB为模型病毒，在无臭氧需求的间歇式系统中研究了臭氧的杀菌效果。利用中空纤维膜对QB的灭活不符合一级动力学规律，研究了中空纤维膜对异养菌和大肠杆菌的去除效果。在某净水厂取水滤液中检测到内源性RNA酶，未检测到直径大于RNA酶的DNA酶，说明采用RNA酶作为模型病毒进行水质控制更为合理。

项目成果

期刊论文数量（31）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Shinichiro OHGAKI: "Handbook of Separation and Purification Technology" Maruzen. 1176 (1993)

Shinichiro OHGAKI：《分离纯化技术手册》丸善。

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神子直之,大垣眞一郎(分担執筆): "環境微生物工学研究法" 技報堂出版, 417 (1993)

Naoyuki Miko、Shinichiro Ogaki（合着）：《环境微生物工程研究方法》Gihodo Publishing，417（1993）

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Katsuo TAKAHASHI, Shinichiro OHGAKI Kenji FUJITA, Naoyuki KAMIKO, Yoshihide KAIYA: "Characteristics of Removal of Bacteria and Viruses by Hollow Fiber Membrane" Proc.27th Ann.Conf., Jpn.Soc.Water Environ.234-235 (1993)

Katsuo TAKAHASHI、Shinichiro OHGAKI Kenji FUJITA、Naoyuki KAMIKO、Yoshihide KAIYA：“中空纤维膜去除细菌和病毒的特性”Proc.27th Ann.Conf.，Jpn.Soc.Water Environ.234-235 (1993)