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Micro-Ecosystem for the Estimation of Micro-Pollutants' Behavior in Water Bodies

用于估计水体中微污染物行为的微生态系统

基本信息

批准号：
63550392
负责人：
OHGAKI Shinichiro
金额：
$ 1.28万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

An open channel was used as a micro-ecosystem. The channel water was circulated by a pump and the head space was covered to be a closed system. Mud was put on the bottom of the channel. RNA coliphage Qbeta was used as model virus in the water-sediment micro-ecosystem to investigate the behavior of viruses in the natural water bodies.1. The inactivation rate of coliphage Qbeta was lower in the water-sediment ecosystem than in the water ecosystem. The Qbeta concentration decreased by 10^<-3> times in 96 hours in the ecosystem without sediment, while no such decrease of the Qbeta concentration was observed in the water-sediment ecosystem.2. Viruses in the water-sediment ecosystem could be divided into two categories, free-form viruses and adsorbed viruses. The free-form viruses are suspended viruses in the water phase as individual particles. The adsorbed viruses are attached viruses onto organic or inorganic suspended solids in the water. The concentration of the adsorbed viruses fluctuates with the sedimentation or resuspension of suspended solids.3. The concentration of adsorbed viruses in the sediment was very high in the water-sediment interface region because of the exchange of solids between the water phase and the sediment. Only 1mm-thick region in the water-sediment interface was turned out to be the high-virus-concentration region under the hydraulic condition of average velocity 11.2cm/s and Reynolds number 1550(turbulent flow).4. The ratio of the adsorbed Qbeta concentration in the sediment(PFU/g) and the concentration in the bulk water(PFU/ml) was 46.5(ml/g).

一个开放的渠道被用作一个微生态系统。通过泵使通道水循环，并将顶部空间覆盖为封闭系统。泥浆被涂在渠道的底部。以RNA大肠杆菌噬菌体Qbeta为模型病毒，研究病毒在水体-沉积物微生态系统中的行为.水体-沉积物生态系统中大肠杆菌噬菌体Q β的灭活率低于水体生态系统。在<-3>无沉积物的生态系统中，Qbeta浓度在96 h内下降了10倍，而在水-沉积物生态系统中没有观察到Qbeta浓度的下降.水-沉积物生态系统中的病毒可分为游离型病毒和吸附型病毒。游离形式病毒是作为单个颗粒悬浮在水相中的病毒。吸附病毒是指病毒附着在水体中的有机或无机悬浮物上。吸附的病毒浓度随悬浮固体的沉降或再悬浮而波动.在水-沉积物界面区域，由于水相和沉积物之间的固体交换，沉积物中吸附的病毒浓度很高。在平均流速为11.2cm/s，雷诺数为1550（紊流）的水力条件下，水-沉积物界面上只有1 mm厚的区域是病毒的高浓度区.沉积物中吸附的Q β浓度（PFU/g）与本体水中的浓度（PFU/ml）之比为46.5（ml/g）。