Interaction between vacuolar H+ ATPase and lymphocytic cholinergic system in regulation of immune function

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We investigated interaction between vacuolar type H+ ATPase (V-ATPase) and lymphocytic cholinergic system in regulation of immune activity. In addition of mediatophore, which is a homooligomer with a 16-kDa subunit homologous to proteolipid subunit c of the V0 sector of V-ATPase, expression of several subunits (a,b,c,d,e) of the V0 sector mRNAs, which is a hydrophobic transmembrane sector of the V-ATPase, were detected in two human leukemic T cell lines CCRF-CCRF-CEM and MOLT-3, and MNLs of human and mouse. T cell activation induced a slight increase in expression of V-ATPase subunits. Anti-mediatophore siRNA decreased mediatophore mRNA expression and release of acetylcholine (ACh) from T cells, without reducing expression of choline acetyltransferase, a synthesizing enzyme of ACh. These findings indiucate that T cells express several subunits of V-ATPase V0 component, and acetylcholine release from T cells is regulated by V-ATPase.