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Phytophagous lady beetle, Epilachana niponica, and its food plants, Cirsium thistles

植食性瓢虫 Epilachana niponica 及其食用植物蓟蓟

基本信息

批准号：
05454008
负责人：
NAKAMURA Koji
金额：
$ 3.52万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

1. Three species of the thistles, Cirsium kagamontanum, C.matumurae var. dubium and C.matsumurae var. matsumurae in the Hokuriku and the adjacent areas were studied on their geographical distribution, morphology, and choromozome numbers.2. A new species, C.ashiuense Yokoyama et T.Shimizu was described. The species is closely related to C.Kagamontanum and is tetraploid (2n=68), while C.kagamontanum is diploid.3. Locations of distribution of C.kagamontanum is confirmed in Ishikawa, Toyama and Niigata Prefectures.4. RFLPs analysis of chloroplast DNA were carried out for 13 species of Cirsium, with Synurus Pungesns as an out-group. phylogeny of the thistles examined was not clarified baxause no variation was detected by the analysis.5. Extract from C.kagamontanum leaves deterred the Asiu population of E.niponica from feeding in the bioassay experiments, confirming the existence of anti-feeding chemicals in the thistle leaves.6. Food preference and adaptability of E.niponica local populations to local thistle species were tested by using the pairs of the beetles and thistles from 5 localities.7. Allozyme analysis was carried out on 17 E.niponica, 1 E.pustulosa and 1 E.yastomii populations, and genetic distances were measured for these populations.8. Multivariate analysis was executed on 8 E.nippnica populations by using 13 morphological characters, and morphological distance was calculated for the populations.

1.蓟属植物中的3种，分别为大蓟、小蓟、小蓟。Dubium和松毛虫C.matSumurae var.本文主要研究内容如下：1.研究了北区及邻近地区松毛虫的地理分布、形态和染色体数目。本文记述了一新种--异尖线虫C.ashiuense Yokyama et T.Shimizu。该种与Kagamontanum亲缘关系较近，为四倍体(2n=68)，而C.kagamontanum为二倍体。在石川县、富山县和新泻县确定了卡格蒙塔菌的分布位置。对13种蓟属植物的叶绿体DNA进行了RFLPS分析，其中SyNurus Pungesns为外群。由于分析没有检测到变异，所研究的蓟的系统发育没有被阐明。在生物测定实验中，卡格蒙丹叶提取物对尼波罗非鱼Asiu种群具有一定的拒食作用，证实了蓟叶中存在拒食化学物质。以5个地区的成对甲虫和蓟为材料，测试了尼波尼察地方种群对当地蓟种的食性和适应性。对17个尼波尼亚、1个化脓性和1个鹅绒委陵菜居群进行了等位酶分析，并测量了这些居群的遗传距离。利用13个形态性状对8个日本龙须草种群进行了多元分析，并计算了种群间的形态距离。