Development of a novel therapy targeting tight-junction protein occludin against hepatitis C

开发针对丙型肝炎的紧密连接蛋白occludin的新疗法

• 批准号: 23659187
• 负责人: CHIBA Hideki
• 金额: $ 2.41万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659187/
• 关键词: タイト結合; C 型肝炎ウィルス; 感染症; 肝癌; トうンスレーショナルリサーチ; 抗体医薬; C型肝炎ウィルス; トランスレーショナルリサーチ; C型肝炎

To prevent and treat Hepatitis C virus (HCV) infection, we generated a novel monoclonal antibody against human occludin. 15 clones of the purified antibodies showed no toxicity in the human hepatocellular carcinoma cell line Huh7.5.1. These antibodies did not inhibit HCV infection in Huh7.5.1 monolayer culture. However, when the fluorescent labeling antibody was applied to the lower chamber, the signal was detected along cell borders of Huh7.5.1 cells cultured in the upper chamber, suggesting that the novel antibody binds to occludin in living cells.

为了预防和治疗丙型肝炎病毒(HCV)感染，我们制备了一种新型的抗人occludin的单抗。15个抗体克隆对人肝癌细胞株Huh7.5.1无毒性作用。这些抗体在Huh7.5.1单层培养中不能抑制丙型肝炎病毒的感染。然而，当荧光标记抗体作用于下室时，在上室培养的Huh7.5.1细胞的细胞边界上检测到信号，表明新抗体与活细胞中的封闭蛋白结合。

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Possible involevement of tight junctions, extracellular matrix and nuclear receptors in epithelial differentiation

紧密连接、细胞外基质和核受体可能参与上皮分化

• 发表时间: 2011
• 期刊: J Biomed Biotechnol
• 作者: Ichikawa-Tomikawa N;Sugimoto K;Satohisa S;Nishiura K;Chiba H
• 通讯作者: Chiba H

Tight-junction protein claudin-6 provokes epithelial polarization and cell-cell junction formation in F9 stem cells

紧密连接蛋白claudin-6在F9干细胞中引发上皮极化和细胞-细胞连接形成

• 发表时间: 2012
• 作者: Kotaro Sugimoto;Naoki Ichikawa-Tomikawa;Risa Kanai;Satoshi Tanaka ;Norimasa Sawada;Hideki Chiba
• 通讯作者: Hideki Chiba

Claudin-4-targeted therapy using Clostridium perfringens enterotoxin for prostate cancer

• DOI: 10.1002/pros.21436
• 发表时间: 2012-03-01
• 期刊: PROSTATE
• 影响因子: 2.8
• 作者: Maeda, Toshihiro;Murata, Masaki;Sawada, Norimasa
• 通讯作者: Sawada, Norimasa

Laminin a1 is essential for mouse cerebellar development.

层粘连蛋白 a1 对于小鼠小脑发育至关重要。

• 发表时间: 2012
• 期刊: Matrix Biol
• 影响因子: 6.9
• 作者: Ichikawa-Tomikawa N;Ogawa J;Douet V;Xu Z;Kamikubo Y;Sakurai T;Kohsaka S;Chiba H;Hattori N;Yamada Y,Arikawa-Hirasawa E
• 通讯作者: Yamada Y,Arikawa-Hirasawa E