Involvement of axon guidance molecules in the formation of early neuronal scaffolds and concomitant swimming behavior in anamuniote vertebrates

轴突引导分子参与无脊椎动物早期神经元支架的形成和伴随的游泳行为

• 批准号: 24650178
• 负责人: MURAKAMI Yasunori
• 金额: $ 2.41万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650178/
• 关键词: 脳・神経; 発生; 行動; 進化; 遺伝子

Vertebrate brain has specific neuronal tracts called the early axonal scaffold, which appear during its early development. These tracts include the longitudinal and commissural axonal bundles such as posterior commissure (pc), which are highly conserved in gnathostomes. We investigated the brain development and gene expression of axon guidance molecules in vertebrates including lamprey, teleosts, amphibians and reptiles, and found that pc was conserved in vertebrates. We further demonstrated that inhibition of Slit2 and Robo2 in Xenopus larvae resulted in the abnormal pc morphology and uncoordinated swimming pattern. Thus, Slit-Robo interaction appears to be involved not only in the formation of pc but also in the establishment of functional brain elements that triggers a swimming behavior. In course of the evolution, Slit-Robo signaling will be contributed to the construction of pc which is a prerequisite for the subsequent development of complex neuronal circuits and functions.

脊椎动物的大脑有特殊的神经束称为早期轴突支架，出现在其早期发育。这些神经束包括纵束和连合轴突束，如后连合（pc），这些轴突束在有颌类中高度保守。我们研究了七鳃鳗、硬骨鱼、两栖类和爬行类等脊椎动物的脑发育和轴突导向分子的基因表达，发现pc在脊椎动物中是保守的。我们进一步证明，在爪蟾幼虫中抑制Slit 2和Robo 2导致异常的pc形态和不协调的游泳模式。因此，Slit-Robo相互作用似乎不仅参与了pc的形成，而且还参与了触发游泳行为的功能性脑元素的建立。在进化过程中，Slit-Robo信号将有助于pc的构建，这是随后复杂神经元回路和功能发展的先决条件。

项目成果

Evolution of the regionalization and patterning of the vertebrate telencephalon: what can we learn from cyclostomes?

• DOI: 10.1016/j.gde.2013.02.008
• 发表时间: 2013-08
• 期刊: Current opinion in genetics & development
• 影响因子: 4
• 作者: F. Sugahara;Y. Murakami;N. Adachi;S. Kuratani

脊椎動物の進化過程における脳領域と神経回路の多様化

脊椎动物进化过程中大脑区域和神经回路的多样化

• 发表时间: 2012
• 作者: 塚野清人;深川真惟;川口将史;鈴木賢一;新居由佳子;高田裕美;村上安則;Y. Murakami;村上安則
• 通讯作者: 村上安則

Reptiles: a new model for brain evo-devo research.

• DOI: 10.1002/jez.b.22484
• 发表时间: 2013-03
• 期刊: Journal of experimental zoology. Part B, Molecular and developmental evolution
• 作者: T. Nomura;Masahumi Kawaguchi;K. Ono;Y. Murakami

生物の科学遺伝特集形態進化のロジックを辿る「脊椎動物の脳の起源と進化」

生物科学遗传学专题：《脊椎动物大脑的起源与进化》追踪形态进化的逻辑

• 发表时间: 2013
• 作者: 鈴木大地;村上安則
• 通讯作者: 村上安則

Toxic effect of 2,3,7,8-tetrachlorodibenzo?dioxin on the peripheral nervous system of developing red seabream (Pagrus major)

2,3,7,8-四氯二苯并二恶英对发育中的真鲷周围神经系统的毒性作用

• 发表时间: 2013
• 期刊: Aquatic toxicology
• 影响因子: 4.5
• 作者: M. Iida;E.Y. Kim;Y. Murakami;Y. Shima;H. Iwata
• 通讯作者: H. Iwata