Construction of systematic tools forgenome analysesto determinegenes responsible for various disease model in rats

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In order to identify genes responsible for phenotypes observed in disease-model rats and determine molecular mechanisms producing those phenotypes, we modified our self-made rat exon capture system, which can easily analyze larger sets of exons in a liquid phase manner. We constructed an effective pipeline to analyze next-generation sequencing data with larger information of polymorphism and gene annotation data in rats. Through combining those systems with linkage analyses, we constructed effective and systematic tools for rat genome analysis. We finally applied this tool to a newly established rat model with unique phenotype, and validated usefulness of our system by collecting candidate variations possibly responsible for its phenotype.