Molecular genetic analysis of atherosclerosis and atrial thrombosis rat model spontaneously induced by hypoactivity

自发性低活动性动脉粥样硬化及心房血栓大鼠模型的分子遗传学分析

• 批准号: 25560368
• 负责人: IMOTO Issei
• 金额: $ 2.5万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25560368/
• 关键词: スポーツ医学; ゲノム; ラット; 遺伝学; 高血圧; 血栓; エピジェネティックメモリー; 次世代シーケンサー

We tried to identify genetic alterations responsible for atherosclerosis and atrial thrombosis with cerebral infarction spontaneously induced by hypoactivity in the SPORTS (Spontaneously Running Tokushima-Shikoku) rat model and molecular mechanisms of preventive effects of exercise on these phenotypes. We modified our self-made rat exon capture system and linkage analysis system, and identified a set of candidate responsible genes using these systems. Genome-wide expression analysis of vascular system also identified candidate genes associated with these phenotypes. In addition, we established systems to determine DNA methylation status and modify genome structure in rats. However, specific gene(s) and molecular mechanisms responsible for those phenotypes were unable to be determined even though using integrated analysis of omics data, suggesting that unknown genomic alterations and/or polygene-environment interactions contribute to acquire these phenotypes in this model.

我们试图确定运动(自发跑步德岛-四国)大鼠模型中动脉粥样硬化和心房血栓形成并自发脑梗塞的遗传改变，以及运动对这些表型的预防作用的分子机制。我们对自制的大鼠外显子捕获系统和连锁分析系统进行了改进，并利用这些系统确定了一组候选负责基因。对血管系统的全基因组表达分析也确定了与这些表型相关的候选基因。此外，我们还建立了确定大鼠DNA甲基化状态和修改基因组结构的系统。然而，即使使用组学数据的综合分析，也无法确定导致这些表型的特定基因(S)和分子机制，这表明在该模型中，未知的基因组改变和/或多基因-环境相互作用有助于获得这些表型。

项目成果

次世代シークエンサー解析により診断したSENDA小児例

通过下一代测序仪分析诊断的 SENDA 儿科病例

• 发表时间: 2013
• 作者: 岡本伸彦;他
• 通讯作者: 他

Early results of omitting completion axillary lymph node dissection in sentinel lymph node metastasis-positive breast cancer patients

前哨淋巴结转移阳性乳腺癌患者省略腋窝淋巴结清扫的早期结果

• DOI: 10.4236/abcr.2013.24021
• 发表时间: 2013
• 期刊: Advances Breast Cancer Res
• 作者: Honda J;Matsuoka H;Hirose C;Nagao T;Y oshida T;T akahashi M;Imoto I;Sasa S
• 通讯作者: Sasa S

Impact of annual BMI gain on obesity development in Japanese 6-year-old nonobese children

BMI 年度增长对日本 6 岁非肥胖儿童肥胖发展的影响

• DOI: 10.1111/ped.12172
• 发表时间: 2013
• 期刊: Pediatr Int
• 影响因子: 1.4
• 作者: Kori H;Sei M;Nakahori Y;Imoto I
• 通讯作者: Imoto I

NF-κB inducing kinase, a central signaling component of the non-canonical pathway of NF-κB, contributes to ovarian cancer progression.

NF-κB诱导激酶是NF-κB非经典途径的中心信号传导成分，有助于卵巢癌的进展。

• DOI: 10.1371/journal.pone.0088347
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Uno M;Saitoh Y;Mochida K;Tsuruyama E;Kiyono T;Imoto I;Inazawa J;Yuasa Y;Kubota T;Yamaoka S
• 通讯作者: Yamaoka S

Replication study and meta-analysis of non-obstructive azoospermia in Japanese populations

日本人群非梗阻性无精子症的复制研究和荟萃分析

• 发表时间: 2013
• 期刊: Biol Reprod
• 影响因子: 3.6
• 作者: Sato Y;Jinam T;Iwamoto T;Yamauchi T;Yamauchi A;Imoto I;Inoue I;Tajima A
• 通讯作者: Tajima A